Cloning, physical mapping and cross-hybridization of the canine adenovirus types 1 and 2 genomes
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P Jouvenne1, M Dion, C Hamelin
1Centre de Recherche en Virologie, Institut Armand-Frappier, Laval-des-Rapides, Québec, Canada.
Gene
|January 1, 1987
Abstract:
Cloned HindIII fragments of the type 1 and type 2 canine adenovirus (canAd) genomes were mapped with respect to the BamHI, EcoRI, HincII, PaeR7 and PstI restriction endonuclease cleavage sites. Considering the large differences found between the canAd-1 and canAd-2 DNA molecules, in terms of number and location of restriction sites, cross-hybridization experiments were performed. Homologous DNA sequences were located on the canAd-1 and canAd-2 physical maps. Both viruses are genetically related to the extent of 57%. Our results confirm the existence of two distinct Ad species in the dog.