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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

94.4K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
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Sanger Sequencing01:57

Sanger Sequencing

762.3K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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RNA-seq03:21

RNA-seq

10.6K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.6K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

11.7K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
11.7K
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

8.4K
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
8.4K

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Related Experiment Video

Updated: Oct 23, 2025

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors
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Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

Published on: September 20, 2016

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Next-Generation Sequencing in Cancer.

S Vinod Nair1, Madhulaxmi2, Gigi Thomas3

  • 1Department of Oral and Maxillofacial Surgery, P.M.S Dental College, Vattapara, Trivandrum, India.

Journal of Maxillofacial and Oral Surgery
|August 19, 2021
PubMed
Summary
This summary is machine-generated.

Next-generation sequencing (NGS) offers comprehensive insights into cancer genomics, transcriptomics, and epigenomics. This technology is crucial for cost-effective molecular diagnosis and advancing cancer research.

Keywords:
CancerCancer researchMolecular diagnosisNGSNext generation sequencing

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Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
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Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing
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Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors
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Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
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Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing
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Area of Science:

  • Genomics and Molecular Biology
  • Cancer Research and Diagnostics

Background:

  • Next-generation sequencing (NGS) technologies, including DNA and RNA sequencing, reveal the genomic, transcriptomic, and epigenomic landscapes of tumors.
  • NGS enables simultaneous screening of diverse genomic abnormalities, such as common and rare variants, insertions, deletions, copy-number variations, and fusion transcripts.

Purpose of the Study:

  • To provide a comprehensive overview of Next-generation sequencing (NGS) technologies.
  • To highlight the applications of NGS in cancer research and molecular diagnostics.

Main Methods:

  • Review of Next-generation sequencing (NGS) principles and capabilities.
  • Discussion of bioinformatics tools for analyzing NGS data.

Main Results:

  • NGS facilitates the simultaneous analysis of multiple genes in a cost-effective manner.
  • NGS has been successfully applied to analyze clinical cancer samples.

Conclusions:

  • NGS, coupled with bioinformatics, enhances our understanding of cancer.
  • NGS is increasingly vital for the molecular diagnosis of various cancers.
  • NGS-based molecular diagnosis offers a powerful tool for personalized cancer care.