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Large three-dimensional cell constructs for tissue engineering.

Jun-Ichi Sasaki1, Gabriela L Abe1, Aonan Li1

  • 1Department of Biomaterials Science, Osaka University Graduate School of Dentistry, Suita, Japan.

Science and Technology of Advanced Materials
|August 19, 2021
PubMed
Summary
This summary is machine-generated.

This study introduces a novel thermo-responsive hydrogel system for creating scaffold-free 3D cell constructs. These constructs enable the regeneration of bone and dental pulp-like tissues, overcoming limitations of traditional tissue engineering scaffolds.

Keywords:
30 Bio-inspired and biomedical materials; 211 Scaffold / Tissue engineering/Drug deliveryCell-based biomaterialbiomimetic materialbone regenerationdental pulp regenerationin vitro tissue engineeringorganoidthermo-responsive hydrogel

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Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Traditional tissue engineering relies on scaffolds, but controlling degradation and byproducts is challenging.
  • Scaffold-free approaches offer potential solutions for improved tissue regeneration.

Purpose of the Study:

  • To develop a novel thermo-responsive hydrogel system for scaffold-free 3D cell construct fabrication.
  • To demonstrate the potential of these constructs for bone and dental pulp tissue regeneration.

Main Methods:

  • Utilizing thermo-responsive hydrogels to form 3D cell constructs with controllable size and morphology.
  • Employing bone marrow-derived stromal stem cells (BMSCs) and dental pulp stem cells (DPSCs).
  • Incorporating endothelial cells to enhance vascularization and bone formation.

Main Results:

  • Scaffold-free 3D constructs from BMSCs formed bone-like tissue with endochondral ossification.
  • Co-culture with endothelial cells promoted bone formation and vascularization within constructs.
  • Freeze-drying improved construct applicability, and DPSCs regenerated pulp-like tissue in a tooth model.

Conclusions:

  • Thermo-responsive hydrogels provide a versatile platform for scaffold-free tissue engineering.
  • This technology facilitates the in vitro fabrication of bone and dental pulp-like tissues with clinical potential.