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Inhibitory effects of ultrasound irradiation on Staphylococcus epidermidis biofilm
Harumi Koibuchi1, Yasutomo Fujii2, Yusuke Sato'o3
1Department of Clinical Laboratory Medicine, Jichi Medical University, 3311-1 Yakushiji Shimotsuke-Shi, Tochigi, 329-0498, Japan. koichan@jichi.ac.jp.
Purpose:
We aimed to investigate whether low-intensity continuous and pulsed wave ultrasound (US) irradiation can inhibit the formation of Staphylococcus epidermidis biofilms, for potential application in the treatment of catheter-related bloodstream infections (CRBSI).
Methods:
S. epidermidis biofilms that formed on the bottom surfaces of 6-well plates were irradiated on the bottom surface using the sound cell incubator system for different intervals of time.
Results:
US irradiation with continuous waves for 24 h notably inhibited biofilm formation (p < 0.01), but the same US irradiation for 12 h had no remarkable effect. Further, double US irradiation with pulsed waves for 20 min inhibited biofilm formation by 33.6%, nearly two-fold more than single US irradiation, which reduced it by 17.9%.
Conclusion:
US irradiation of a lower intensity (ISATA = 6-29 mW/cm2) than used in a previous study and lower than recommended by the Food and Drug Administration shows potential for preventing CRBSI caused by bacterial biofilms.
