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Water-Compatible Fluorescent Molecularly Imprinted Polymers.

Huiqi Zhang1

  • 1State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Functional Polymer Materials (Ministry of Education), Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), and College of Chemistry, Nankai University, Tianjin, P. R. China. zhanghuiqi@nankai.edu.cn.

Methods in Molecular Biology (Clifton, N.J.)
|August 19, 2021
PubMed
Summary

Researchers developed water-compatible molecularly imprinted polymers (MIPs) for detecting antibiotics in complex biological fluids. These hydrophilic fluorescent MIP nanoparticles offer selective and sensitive optosensing capabilities in undiluted serums.

Keywords:
Complex biological samplesControlled/“living” radical precipitation polymerizationFluorescent sensorsHydrophilic polymer brushesMolecularly imprinted polymersNanoparticlesWater-compatible

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Area of Science:

  • Polymer Chemistry
  • Analytical Chemistry
  • Biomedical Engineering

Background:

  • Molecularly imprinted polymers (MIPs) are crucial for selective analyte recognition.
  • Developing MIPs for direct use in complex biological samples like undiluted serum remains challenging.
  • Water-compatibility and fluorescence are desirable traits for advanced sensing applications.

Purpose of the Study:

  • To prepare hydrophilic fluorescent MIP nanoparticles compatible with complex biological samples.
  • To enable direct, selective, sensitive, and accurate optosensing of small organic analytes.
  • To demonstrate the application of these MIPs for antibiotic detection in undiluted animal serums.

Main Methods:

  • Controlled grafting of hydrophilic polymer brushes onto MIP particle surfaces.
  • Two synthetic approaches: "two-step" and "one-step" for nanoparticle preparation.
  • Optosensing assays using fluorescent MIP nanoparticles for tetracycline detection.

Main Results:

  • Successfully synthesized water-compatible hydrophilic fluorescent MIP nanoparticles.
  • Demonstrated direct and selective recognition of tetracycline in undiluted bovine and porcine serums.
  • Achieved sensitive and accurate optosensing of the antibiotic, showcasing practical applicability.

Conclusions:

  • Hydrophilic polymer brush grafting is an effective strategy for creating water-compatible MIPs.
  • The developed MIP nanoparticles are suitable for direct analysis of complex biological samples.
  • These MIPs offer a promising platform for sensitive and selective optosensing of antibiotics and potentially other small molecules.