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Distinguishing normal and inflammatory models by viscosity changes with sensitively mitochondrial-trackable
Chen Geng1, Jingting Zhan1, Xinya Hao1
1Guangxi Key Laboratory of Electrochemical Energy Materials, Institute of Optical Materials and Chemical Biology, School of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi 530004, PR China.
Summary
Researchers developed a new fluorescent probe (HVM) to detect changes in mitochondrial viscosity, aiding in disease identification. This probe offers excellent optical properties and biocompatibility for biological applications.
Area of Science:
- Biochemistry
- Molecular Biology
- Biomedical Engineering
Background:
- The biological microenvironment is crucial for cellular functions, and its alterations are linked to diseases.
- Microenvironment viscosity is a key parameter, making its measurement a significant research area.
- Mitochondrial dysfunction is implicated in various pathological conditions.
Purpose of the Study:
- To construct a novel red-emitting fluorescent probe (HVM) for sensing viscosity changes in the biological microenvironment.
- To investigate the probe's utility in identifying abnormal mitochondrial viscosity associated with disease states.
- To provide a facile synthetic method for a highly sensitive viscosity sensor.
Main Methods:
- Synthesis of a novel red-emitting fluorescent probe (HVM).
- Characterization of HVM's optical properties, including Stokes shift and viscosity sensitivity.
- Evaluation of HVM's biocompatibility and cytotoxicity.
- Application of HVM to differentiate normal and inflammatory biological models based on viscosity changes.
Main Results:
- HVM exhibits excellent optical properties: a large Stokes shift (160 nm) and high viscosity sensitivity (195-fold).
- The probe demonstrates high photostability, low cytotoxicity, and excellent biocompatibility.
- HVM successfully distinguished between normal and inflammatory biological models by detecting viscosity variations.
Conclusions:
- The developed HVM probe is a valuable tool for monitoring biological microenvironment viscosity, particularly in mitochondria.
- HVM's unique properties enable the identification of disease-related viscosity changes.
- This study presents a convenient route to a versatile viscosity sensor with significant potential in biological and medical research.
