Insight into the mechanism of nitritation establishment through external fermented sludge addition

In this study, a novel strategy for nitritation establishment through external fermented sludge addition is proposed. The nitrifying activities under various fermentation times were assessed; batch tests results revealed that the decay rate of nitrite oxidizing bacteria (0.53 ± 0.08 d^-1) was much higher than that of ammonia oxidizing bacteria (0.15 ± 0.04 d^-1), during alkaline fermentation with high endogenous free ammonia concentrations (0.57-138.79 mg·L^-1). The long-term effects of fermented sludge addition were constantly monitored in an anaerobic/oxic/anoxic sequencing batch reactor. During 60 days of operation, the nitrite accumulation rate reached above 90% and could be maintained stably. Total inorganic nitrogen removal achieved 94.7%, with an average external sludge reduction rate of 61.3%, after incorporation of the sludge addition. Illumina MiSeq sequencing demonstrated that nitrite oxidizing bacteria were successfully eliminated and the relative abundance of ammonia oxidizing bacteria remained at 0.39%, resulting in nitrite accumulation.