Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Complex Assembly02:41

Protein Complex Assembly

13.9K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
13.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Silver diamine fluoride versus conventional restorative treatment for dental caries in primary teeth of children: a meta-analysis.

Quintessence international (Berlin, Germany : 1985)·2026
Same author

Identification and Characterization of Novel Anti-inflammatory and Hepatoprotective Properties of Dual-Function Peptides Derived from Jinhua Ham: A Study Integrating Computational Modeling with the cGAS-STING Pathway.

Journal of agricultural and food chemistry·2026
Same author

Sodium carboxymethylcellulose-sodium caseinate complex hydrocolloid enhances the oil barrier properties of batter-coated deep-fried pork.

Journal of the science of food and agriculture·2026
Same author

Myofibrillar Fragmentation and Cytoskeletal Protein Degradation: A Mechanism Driven by Ultra-High Pressure Processing to Tenderize Post-Mortem Goose Meat.

Journal of texture studies·2026
Same author

Early ICU glycemic trajectory phenotypes predict short- and long-term mortality in critically ill patients: a dual-database cohort study.

Metabolism open·2026
Same author

Freestanding Flexible Vortex Tubes Integrated With Ferroelectric Transistor.

Advanced materials (Deerfield Beach, Fla.)·2026

Related Experiment Video

Updated: Oct 23, 2025

Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides
07:26

Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides

Published on: November 21, 2013

13.1K

Self-Assembled Pea Protein Isolate Nanoparticles with Various Sizes: Explore the Formation Mechanism.

Xiao-Long Li1,2, Qiu-Tao Xie3, Wen-Jie Liu1,2

  • 1Engineering Research Center of Bio-Process, Ministry of Education, Hefei University of Technology, 193 Tunxi Road, Hefei, Anhui 230009, P. R. China.

Journal of Agricultural and Food Chemistry
|August 20, 2021
PubMed
Summary

Pea protein isolate nanoparticles (PPINs) were created using potassium metabisulfite. This method offers a simple, economical way to produce PPINs with controlled size and stability.

Keywords:
disulfide bondhydrophobic interactionsnanoparticlespea protein isolatepotassium metabisulfite

More Related Videos

Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures
08:15

Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures

Published on: June 26, 2020

4.4K
Facile Protocol for the Synthesis of Self-assembling Polyamine-based Peptide Amphiphiles PPAs and Related Biomaterials
08:55

Facile Protocol for the Synthesis of Self-assembling Polyamine-based Peptide Amphiphiles PPAs and Related Biomaterials

Published on: June 25, 2018

8.2K

Related Experiment Videos

Last Updated: Oct 23, 2025

Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides
07:26

Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides

Published on: November 21, 2013

13.1K
Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures
08:15

Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures

Published on: June 26, 2020

4.4K
Facile Protocol for the Synthesis of Self-assembling Polyamine-based Peptide Amphiphiles PPAs and Related Biomaterials
08:55

Facile Protocol for the Synthesis of Self-assembling Polyamine-based Peptide Amphiphiles PPAs and Related Biomaterials

Published on: June 25, 2018

8.2K

Area of Science:

  • Food Science and Technology
  • Materials Science
  • Biochemistry

Background:

  • Pea protein isolate is a valuable source of proteins for various applications.
  • Nanoparticle fabrication is crucial for enhancing the functional properties of food ingredients.
  • Developing cost-effective and simple methods for nanoparticle production is essential.

Purpose of the Study:

  • To develop a simple and economical method for preparing pea protein isolate nanoparticles (PPINs).
  • To investigate the effect of potassium metabisulfite concentration on PPIN formation and characteristics.
  • To elucidate the self-assembly mechanism and stabilizing forces within PPINs.

Main Methods:

  • Pea protein isolate was treated with varying concentrations of potassium metabisulfite (K2S2O5).
  • Nanoparticle size, ζ-potential, and distribution were analyzed using dynamic light scattering.
  • Fourier-transform infrared spectroscopy (FTIR) was used to study secondary structure changes.
  • Dissociation tests were performed to identify the forces stabilizing the nanoparticles.

Main Results:

  • PPINs were successfully synthesized via self-assembly after disulfide bond disruption by K2S2O5.
  • Increasing K2S2O5 concentration (2-8 mM) led to larger PPINs (124.7-297.5 nm) with improved ζ-potentials (-32.2 to -35.8 mV).
  • Hydrophobic interactions, disulfide bonds, and hydrogen bonds were identified as key stabilizing forces for PPINs.

Conclusions:

  • Potassium metabisulfite provides an effective means to fabricate pea protein isolate nanoparticles.
  • The method allows for control over nanoparticle size and stability through K2S2O5 concentration.
  • The findings offer a simple, economical approach for producing functional protein-based nanoparticles.