Anatomical study of enkephalin gene expression in the rat forebrain following haloperidol treatment

The effect of haloperidol treatment on preproenkephalin A (PPA) gene expression in the rat forebrain was anatomically studied by using an in situ hybridization procedure. The PPA mRNA was detected in sections of control rats and of rats having received an i.p. injection of haloperidol for 14 or 21 days. Sections were incubated with rat PPA cDNA labeled with 32P or 35S, exposed with X-ray film and dipped in Ilford K-5 emulsion. The results showed that haloperidol treatment did not modify the number of cells expressing the PPA gene in the caudate-putamen, the nucleus accumbens, the septum and the olfactory tubercle. In contrast, the PPA mRNA content was increased in the neurons of the caudate-putamen and nucleus accumbens, but unchanged in other areas. These results demonstrate that haloperidol acts by increasing PPA mRNA content selectively in striatal neurons already expressing the PPA gene.