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Related Experiment Videos

Insertion sequence IS2 associated with int-constitutive mutants of bacteriophage lambda.

W Pilacinski, E Mosharrafa, R Edmundson

    Gene
    |January 1, 1977
    PubMed
    Summary

    Bacteriophage lambda int-c mutations lead to increased int gene expression. These mutations either disable a transcription terminator or introduce a new promoter, making int gene expression constitutive.

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    Gene·2001

    Area of Science:

    • Molecular Biology
    • Genetics
    • Microbiology

    Background:

    • Bacteriophage lambda integrates into the host genome via a site-specific recombination mechanism.',
    • The int gene encodes the integrase protein, essential for lambda integration.',
    • Regulation of int gene expression is crucial for controlling lysogeny and lytic cycles.

    Purpose of the Study:

    • To investigate bacteriophage lambda mutations conferring elevated constitutive expression of the int gene (int-c mutations).
    • To elucidate the molecular mechanisms underlying constitutive int gene expression in these mutants.

    Main Methods:

    • Genetic mapping of int-c mutations.
    • Analysis of IS2 insertion sequences in specific mutant classes.
    • Inference of transcriptional regulation mechanisms based on mutation location and known regulatory elements (pI-tI leader, cII, cIII products).

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    Main Results:

    • Two classes of int-c mutations were identified.
    • Class one mutations, mapping between b538 and bio386, appear to be point mutations inactivating the tI terminator signal.
    • Class two mutations involve IS2 insertion in orientation II between the int gene and the b538 endpoint, suggesting a novel promoter.
    • Most int-c mutations are located within the xis gene, with one exception (int-c548) possibly between int and xis.

    Conclusions:

    • Point mutations in class one directly affect the pI-tI leader RNA, leading to constitutive int expression independent of cII and cIII.
    • IS2 insertion in class two mutants creates a new constitutive promoter, driving int transcription.
    • These findings provide insights into the complex transcriptional regulation of the bacteriophage lambda int gene.