Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Urine Studies I: Urinalysis

Urine Studies I: Urinalysis

Urinalysis is a widely used diagnostic test that analyzes urine's physical, chemical, and microscopic characteristics. Healthcare providers use it to detect and monitor various health conditions, including renal disease, urinary tract infections (UTIs), diabetes, and metabolic or systemic disorders.Components of UrinalysisUrinalysis consists of three primary components: physical, chemical, and microscopic examination. Each provides unique insights into the urine sample and, by extension, the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Operational checks for real-time urinary biomarker measurements for assessment of the quality of deceased donor kidneys.

Scientific reports·2026

Same author

Biomarkers of inflammation and risk of adverse events in patients with cirrhosis.

Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·2026

Same author

Associations between exposure to polycyclic aromatic hydrocarbons and biomarkers of acute kidney injury among Chilean agricultural workers.

International journal of hygiene and environmental health·2026

Same author

Shared multicellular injury programs of acute and chronic kidney disease enable mechanistic patient stratification.

medRxiv : the preprint server for health sciences·2026

Same author

Risk and protective factors for uveal melanoma: Evidence for a protective role of autoimmune and immune-mediated conditions.

Journal of the American Academy of Dermatology·2026

Same author

Concordance of Prebiopsy and Postbiopsy Diagnosis in Hospitalized Patients with Acute Kidney Injury.

Kidney360·2026

Search research articles

Related Experiment Video

Updated: Oct 23, 2025

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Published on: April 23, 2019

Sample Processing and Stability for Urine Biomarker Studies.

Crystal Chang¹, Wassim Obeid¹, Heather Thiessen-Philbrook¹

¹Division of Nephrology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

The Journal of Applied Laboratory Medicine

|August 20, 2021

Summary

This summary is machine-generated.

Urine sample processing and storage methods impact urinary biomarker measurements. Some biomarkers, like IL-6 and NGAL, remain stable across various conditions, aiding research despite preanalytical variations.

Keywords:

acute kidney injury biospecimen handling biospecimen storage protein stability urinalysis

More Related Videos

One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure

One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure

Published on: June 25, 2010

Integration of Miniaturized Solid Phase Extraction and LC-MS/MS Detection of 3-Nitrotyrosine in Human Urine for Clinical Applications

Integration of Miniaturized Solid Phase Extraction and LC-MS/MS Detection of 3-Nitrotyrosine in Human Urine for Clinical Applications

Published on: July 14, 2017

Related Experiment Videos

Last Updated: Oct 23, 2025

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Published on: April 23, 2019

One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure

One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure

Published on: June 25, 2010

Integration of Miniaturized Solid Phase Extraction and LC-MS/MS Detection of 3-Nitrotyrosine in Human Urine for Clinical Applications

Integration of Miniaturized Solid Phase Extraction and LC-MS/MS Detection of 3-Nitrotyrosine in Human Urine for Clinical Applications

Published on: July 14, 2017

Area of Science:

Biomarker discovery and validation
Clinical chemistry and diagnostics
Urine sample preanalytical variables

Background:

Standardized urine sample processing and storage are crucial for reliable biomarker research.
Nonideal preanalytical handling is common, particularly during public health crises like the COVID-19 pandemic.
Systematic comparisons of urine sample handling methods are lacking.

Purpose of the Study:

To compare the effects of different short-term urine storage and processing methods on urinary biomarker measurements.
To identify biomarkers that are robust to preanalytical variations.
To provide guidance for optimal urine sample handling in research settings.

Main Methods:

Spot urine samples were collected from 20 post-cardiac surgery patients.
Three preanalytical conditions were tested: 48-hour storage at 4°C, 48-hour storage at 25°C, followed by freezing at -80°C, and immediate freezing at -80°C without centrifugation.
Concentrations of 18 urinary biomarkers were measured using Meso-Scale Device assays.

Main Results:

Most biomarker measurements were stable after temporary 4°C storage.
Interleukin-6 (IL-6), IL-8, kidney injury molecule-1 (KIM-1), monocyte chemoattractant protein-1 (MCP-1), chitinase-3-like protein 1 (YKL-40), epidermal growth factor (EGF), and neutrophil gelatinase-associated lipocalin (NGAL) were stable across all tested conditions.
Interleukin-12p70 (IL-12p70) and IL-4 showed significant differences across all conditions.

Conclusions:

Several urinary biomarkers demonstrate stability despite variations in preanalytical techniques.
IL-6, IL-8, KIM-1, MCP-1, YKL-40, EGF, and NGAL are reliable biomarkers even with nonideal sample handling.
These findings support the reliable investigation of specific biomarkers under challenging preanalytical conditions.