Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Types of Building Separation Joints

Types of Building Separation Joints

Building separation joints divide large or complex building structures into smaller, discrete units that can move independently. These joints are categorized into three types: volume-change joints, settlement joints, and seismic separation joints.
Volume-change joints address the effects of expansion and contraction due to temperature and moisture variations. They are strategically placed at discontinuities in a building's mass where cracking is most likely and are spaced about 150 to 200...

Acid Attack on Concrete

Acid Attack on Concrete

When acids come into contact with concrete, they initiate a chemical reaction that dissolves the hydrated cement paste. This process leads to softening and structural weakening of the concrete. This issue is commonly observed in environments such as chimneys, sewers, and industrial settings. The severity of the damage increases as the pH of the water interacting with the concrete drops below 6.5. In particular, a pH under 4.5 can cause significant concrete damage.
The rate at which hydrogen...

Masonry

Masonry

Masonry, known for its strength, durability, and aesthetic versatility, encompasses construction with solid stone or man-made units like bricks, clay tiles, terra cotta, and concrete blocks, combined to form structures like walls, floors, and arches. These units are placed in a systematic fashion, known as coursing, and are bound together using mortar—a mixture typically made of water, cement, and sand.
The process of building with masonry is hands-on and can be executed with basic tools....

Placing Concrete

Placing Concrete

The concrete is placed as close as possible to its final position to avoid segregation. The placed concrete is then fully compacted to expel the entrapped air, and the next layer of concrete is laid while the underlying layer is still in the plastic state. The rate at which concrete is placed and compacted is kept equal.
While placing concrete, care is taken to ensure that the concrete is laid in uniform layers, and hand shoveling and moving concrete using poker vibrators is avoided. Also,...

Protein Complex Assembly

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...

Deleterious Substances in Aggregate

Deleterious Substances in Aggregate

Deleterious substances in aggregates can be detrimental to the quality and durability of concrete. These substances include organic impurities like loam, which interfere with cement hydration and are usually present in the sand. These prevent a good bond between aggregate and cement paste. Organic impurities can be detected using the colorimetric test, where the darkness of a solution after agitation indicates the level of organic content.
Another type of impurity is clay and fine material that...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

WNT7B drives a program for pancreatic cancer subtype switching and progression.

iScience·2026

Same author

Hepatocellular carcinoma-linked <i>AXIN1</i> mutations drive low Wnt/β-catenin activity enabling niche-independent growth and YAP/TAZ signaling.

iScience·2026

Same author

BRAF<sup>V600E</sup> augments WNT signaling in colorectal cancer via aberrant DNA methylation.

iScience·2025

Same author

BRAF<sup>V600E</sup> maintains the CpG island methylator phenotype, and DNA methylation of PRC2 targets genes in colon cancer.

iScience·2025

Same author

Mechanistic insights into Wnt-β-catenin pathway activation and signal transduction.

Nature reviews. Molecular cell biology·2025

Same author

Correction: USP10 drives cancer stemness and enables super-competitor signalling in colorectal cancer.

Oncogene·2025

Search research articles

Related Experiment Video

Updated: Oct 23, 2025

Research and Development of High-performance Explosives

Research and Development of High-performance Explosives

Published on: February 20, 2016

Building a complex for destruction.

Anton J Venhuizen¹, Felix van der Krift¹, Madelon M Maurice¹

¹Center for Molecular Medicine, University Medical Center Utrecht, the Netherlands; Oncode Institute, the Netherlands.

|August 20, 2021

Summary

This summary is machine-generated.

Researchers reconstituted the beta-catenin destruction complex in vitro. This study clarifies how its core components, including cancer-related mutations, affect beta-catenin turnover and tumor suppression.

More Related Videos

Laboratory Drop Towers for the Experimental Simulation of Dust-aggregate Collisions in the Early Solar System

Laboratory Drop Towers for the Experimental Simulation of Dust-aggregate Collisions in the Early Solar System

Published on: June 5, 2014

Laser-heating and Radiance Spectrometry for the Study of Nuclear Materials in Conditions Simulating a Nuclear Power Plant Accident

Laser-heating and Radiance Spectrometry for the Study of Nuclear Materials in Conditions Simulating a Nuclear Power Plant Accident

Published on: December 14, 2017

Related Experiment Videos

Last Updated: Oct 23, 2025

Research and Development of High-performance Explosives

Research and Development of High-performance Explosives

Published on: February 20, 2016

Laboratory Drop Towers for the Experimental Simulation of Dust-aggregate Collisions in the Early Solar System

Laboratory Drop Towers for the Experimental Simulation of Dust-aggregate Collisions in the Early Solar System

Published on: June 5, 2014

Laser-heating and Radiance Spectrometry for the Study of Nuclear Materials in Conditions Simulating a Nuclear Power Plant Accident

Laser-heating and Radiance Spectrometry for the Study of Nuclear Materials in Conditions Simulating a Nuclear Power Plant Accident

Published on: December 14, 2017

Area of Science:

Biochemistry
Molecular Biology
Cancer Research

Background:

The beta-catenin destruction complex is crucial for regulating cellular levels of beta-catenin.
Dysregulation of this complex is implicated in various cancers, particularly those involving the Wnt signaling pathway.
Understanding the complex's precise mechanisms is vital for developing targeted cancer therapies.

Purpose of the Study:

To reconstitute the beta-catenin destruction complex in vitro.
To investigate the functional roles of full-length and mutated core components in beta-catenin turnover.
To elucidate the molecular mechanisms underlying the complex's tumor suppressor activity.

Main Methods:

In vitro reconstitution of the beta-catenin destruction complex using purified proteins.
Biochemical assays to measure beta-catenin degradation rates.
Analysis of the impact of specific mutations on complex assembly and function.

Main Results:

Successfully reconstituted a functional beta-catenin destruction complex in vitro.
Demonstrated distinct contributions of full-length and mutated components to beta-catenin turnover.
Identified key interactions and regulatory steps within the complex.

Conclusions:

The study provides a detailed mechanistic understanding of the beta-catenin destruction complex.
Findings highlight the importance of specific protein domains and cancer-associated mutations in regulating beta-catenin stability.
This research advances knowledge of a critical tumor suppressor pathway and offers insights for cancer drug development.