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Nanopore chip with self-aligned transverse tunneling junction for DNA detection
Yuan Wang1, Joshua Sadar1, Ching-Wei Tsao2
1Department of Physics, Arizona State University, Tempe, Arizona, 85287, United States.
Biosensors & Bioelectronics
|August 20, 2021
Summary
Researchers developed a new method for nanopore sequencing using self-aligned transverse tunneling junctions. This technique improves DNA detection and offers a reproducible platform for future sequencing advancements.
Area of Science:
- Nanotechnology
- Biophysics
- Materials Science
Background:
- Quantum tunneling offers potential for enhanced signal quality and resolution in nanopore sequencing.
- Integration of tunneling junctions in nanopores has faced challenges with precision and reproducibility.
Purpose of the Study:
- To develop a novel strategy for constructing nanopore devices with self-aligned transverse tunneling junctions.
- To enable reproducible and high-yield correlated detection of translocating DNA using tunneling junctions.
Main Methods:
- Utilized feedback-controlled electrochemical processes within a confined nanoscale space.
- Fabricated nanopore devices with self-aligned transverse tunneling junctions on a nanofluidic chip.
Main Results:
- Achieved high-yield (>93%) correlated detection of translocating DNA from both ionic and tunneling channels.
- Observed enriched event rates and detected events from non-translocating DNA interacting with electrodes.
Conclusions:
- The developed platform provides an accessible and reproducible method for tunneling-based DNA detection.
- Addresses existing challenges in nanopore sequencing, paving the way for future optimizations and sequencing applications.
