Related Experiment Video
Updated: Oct 23, 2025
10:28
Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
Published on: April 14, 2015
33.4K
Detection of Long Noncoding RNA Expression by Real-Time PCR
Rui Huang1,2, Yihao Wang1,2, Yaqi Deng1,2
1Department of Ophthalmology, Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China.
Methods in Molecular Biology (Clifton, N.J.)
|August 21, 2021
Summary
Quantifying long noncoding RNA (lncRNA) expression is crucial for understanding its biological roles. This guide details using real-time PCR for accurate and efficient lncRNA analysis.
Area of Science:
- Molecular Biology
- Genomics
- RNA Biology
Background:
- Long noncoding RNAs (lncRNAs) are newly identified RNA transcripts with vital biological functions.
- lncRNA expression patterns differ across tissues and conditions, necessitating precise quantification methods.
Purpose of the Study:
- To provide a method for the accurate quantification of long noncoding RNA (lncRNA) expression.
- To detail the application of real-time PCR for analyzing lncRNA abundance.
Main Methods:
- Real-time PCR (Polymerase Chain Reaction) analysis was employed.
- The protocol focuses on determining the identity and quantity of specific lncRNAs.
Main Results:
- Real-time PCR offers a convenient and time-efficient approach for lncRNA measurement.
- The method allows for accurate assessment of lncRNA expression levels.
Conclusions:
- Accurate lncRNA quantification is essential for research in various biological and medical fields.
- Real-time PCR is presented as a practical technique for analyzing lncRNA expression profiles.
Related Concept Videos
02:57Real Time RT-PCR
62.4K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
The real-time quantification of the number of amplified products is...
62.4K
02:24Ribosome Profiling
3.7K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.7K