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CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

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Related Experiment Video

Updated: Oct 23, 2025

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

Genetic Editing of Long Noncoding RNA Using CRISPR/Cas9 Technology.

Kristina Larter¹, Bin Yi¹, Yaguang Xi²

¹Department of Genetics and Stanley S. Scott Cancer Center, Louisiana State University Health Sciences Center, New Orleans, LA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|August 21, 2021

Summary

This study introduces a CRISPR/Cas9 method to knock down long noncoding RNAs (lncRNAs). This genetic editing tool helps uncover the functions of these crucial RNA molecules in various biological processes.

Keywords:

CRISPR/cas9 Genetic editing Knockdown Long noncoding RNA

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Last Updated: Oct 23, 2025

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

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Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

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Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

Published on: March 1, 2019

Area of Science:

Genomics
Molecular Biology
RNA Biology

Background:

Long noncoding RNAs (lncRNAs) are abundant genomic transcripts with vital roles in physiology and pathology.
Understanding lncRNA function is hindered by limitations in current genetic manipulation techniques.
CRISPR/Cas9 technology has shown promise for lncRNA functional screening.

Purpose of the Study:

To present a detailed protocol for lncRNA knockdown using CRISPR/Cas9.
To facilitate in vitro functional studies of lncRNAs.
To overcome challenges in lncRNA genetic manipulation.

Main Methods:

Utilizing CRISPR/Cas9 gene editing technology.
Implementing a specific protocol for lncRNA knockdown.
Performing experiments in an in vitro setting.

Main Results:

A feasible protocol for targeted lncRNA knockdown was established.
The method enables efficient genetic editing of lncRNAs.
The protocol facilitates further investigation into lncRNA functions.

Conclusions:

CRISPR/Cas9 is an effective tool for lncRNA knockdown.
This protocol provides a valuable resource for lncRNA functional genomics.
Advancing lncRNA research through improved genetic editing strategies.