Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Interference01:23

RNA Interference

26.8K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.8K
Viruses with RNA Genomes01:29

Viruses with RNA Genomes

256
RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
256
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

13.8K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
13.8K
Types of RNA01:23

Types of RNA

69.7K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
69.7K
Experimental RNAi02:15

Experimental RNAi

6.5K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.5K
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

17.3K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
17.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Genetic structure of X-STR loci in Bai, Dai and Yi ethnic groups and their affinity with five major populations of China].

Yi chuan = Hereditas·2009
Same author

Suppression of lung cancer in murine model: treated by combination of recombinant human endostsatin adenovirus with low-dose cisplatin.

Journal of experimental & clinical cancer research : CR·2009
Same author

[Researches on cloning and expression of the gene encoding Schistosoma japonicum tetraspanins extracellular loop 2 and its immunogenicity].

Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition·2009
Same author

Asparanin A induces G(2)/M cell cycle arrest and apoptosis in human hepatocellular carcinoma HepG2 cells.

Biochemical and biophysical research communications·2009
Same author

Pharmacophore modeling study based on known spleen tyrosine kinase inhibitors together with virtual screening for identifying novel inhibitors.

Bioorganic & medicinal chemistry letters·2009
Same author

[Application of NIR spectroscopy to multiple gas components identification].

Guang pu xue yu guang pu fen xi = Guang pu·2009

Related Experiment Video

Updated: Oct 23, 2025

Identification of Circular RNAs using RNA Sequencing
08:25

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

12.4K

Characterization of Circular RNAs.

Yang Zhang1, Li Yang2,3, Ling-Ling Chen4,5

  • 1Department of Medicine and Pathology, Cancer Research Institute, Beth Israel Deaconess Cancer Center, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|August 21, 2021
PubMed
Summary
This summary is machine-generated.

Circular RNAs (circular RNAs) are abundant in many species and have unique closed-loop structures. This study presents a detailed pipeline for identifying and validating these important RNA molecules, including circular intronic RNAs (ciRNAs) and exonic circular RNAs (circRNAs).

Keywords:
Circular RNAsDivergent PCRNorthern blotPAGERNA fractionationRNase RciRNAscircRNAs

More Related Videos

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
10:27

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

1.7K
Use of Alu Element Containing Minigenes to Analyze Circular RNAs
13:10

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

7.5K

Related Experiment Videos

Last Updated: Oct 23, 2025

Identification of Circular RNAs using RNA Sequencing
08:25

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

12.4K
In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
10:27

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

1.7K
Use of Alu Element Containing Minigenes to Analyze Circular RNAs
13:10

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

7.5K

Area of Science:

  • Molecular Biology
  • Genomics
  • RNA Biology

Background:

  • Circular RNAs (circular RNAs) are increasingly recognized as prevalent and functionally significant molecules in eukaryotic transcriptomes.
  • Unlike linear RNAs, circular RNAs possess a stable, covalently closed structure lacking 5' caps and 3' tails, necessitating specialized detection methods.
  • Previous research has indicated the presence of circular RNAs across diverse species, from fruit flies to humans, highlighting their conserved nature.

Purpose of the Study:

  • To present a comprehensive and detailed pipeline for the robust identification and characterization of circular RNAs.
  • To provide a validated methodology applicable to different types of circular RNAs, including circular intronic RNAs (ciRNAs) and exonic circular RNAs (circRNAs).

Main Methods:

  • Development of a specific bioinformatic and experimental pipeline tailored for circular RNA detection.
  • Application of the pipeline to analyze RNA samples, focusing on distinguishing circular RNAs from linear RNA contaminants.
  • Utilizing established molecular biology techniques for RNA isolation, library preparation, and sequencing.

Main Results:

  • The described pipeline successfully enabled the characterization of circular RNAs in human transcriptomes.
  • The methodology proved effective in identifying both circular intronic RNAs (ciRNAs) derived from intron lariats and canonical circular RNAs (circRNAs) formed from back-spliced exons.
  • Validation of the pipeline demonstrates its reliability for studying diverse circular RNA populations.

Conclusions:

  • The developed pipeline offers a reliable method for the comprehensive characterization of circular RNAs.
  • This methodology is crucial for advancing the study of circular RNAs, including ciRNAs and circRNAs, in various biological contexts.
  • The findings underscore the importance of specialized techniques for accurately analyzing the complex landscape of circular RNA molecules.