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Related Experiment Video

Updated: Oct 23, 2025

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

Published on: July 9, 2021

Exploration of Circular RNA Interactomes by RNA Pull-Down Method.

Mengshi Wu¹, Dan Peng¹, Xiaomin Zhong²

¹Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Center for Stem Cell Biology and Tissue Engineering, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, China.

Methods in Molecular Biology (Clifton, N.J.)

|August 21, 2021

Summary

This summary is machine-generated.

This study details an RNA pull-down protocol to identify circular RNA (circRNA) interactomes. This method helps uncover the functions and mechanisms of these important regulatory molecules.

Keywords:

Circular RNA RNA pull-down circRNA interactomes

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Last Updated: Oct 23, 2025

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

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Published on: July 9, 2021

An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA

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Published on: February 17, 2023

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

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Published on: July 22, 2016

Area of Science:

Molecular Biology
Genetics
Biochemistry

Background:

Circular RNAs (circRNAs) are RNA molecules formed by back-splicing of pre-mRNA.
circRNAs are recognized as key regulators in physiological and pathological processes.
The specific biological functions and mechanisms of circRNAs are not fully understood.

Purpose of the Study:

To provide a detailed protocol for RNA pull-down.
To enable the capture and identification of circRNA interactomes.
To facilitate further characterization of circRNA functions and mechanisms.

Main Methods:

RNA pull-down assay.
Enrichment of circRNAs and their interacting partners.
Identification of circRNA-binding molecules.

Main Results:

A detailed protocol for RNA pull-down is presented.
The method allows for specific and efficient enrichment of circRNAs.
The protocol facilitates the identification of proteins and other molecules interacting with circRNAs.

Conclusions:

The described RNA pull-down protocol is effective for studying circRNA interactomes.
This method is valuable for elucidating the functional roles of circRNAs.
Further research into circRNA functions can be advanced using this technique.