Jove
Visualize
Contact Us

Related Concept Videos

In Vitro Fertilization01:24

In Vitro Fertilization

566
In vitro fertilization (IVF) is a form of assisted reproductive technology where an egg is fertilized with sperm in a controlled laboratory environment before transferring the resulting embryo into the uterus. This process is designed to help individuals and couples experiencing difficulties conceiving.
The IVF process begins with ovarian stimulation, during which reproductive endocrinologists prescribe hormonal medications to stimulate the ovaries to produce multiple eggs instead of the single...
566

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Histomorphometric Analysis of the Endometrium of Jennies (<i>Equus asinus</i>) and Mares (<i>Equus caballus</i>) in Estrus: Anatomical Differences and Possible Reproductive Implications.

Animals : an open access journal from MDPI·2026
Same author

Effect of silver nanoparticles on donkey sperm parameters and ultrastructure.

Reproduction in domestic animals = Zuchthygiene·2024
Same author

Selection and Conservation of Gametes and Embryos for Improving Assisted Reproductive Technologies.

Animals : an open access journal from MDPI·2023
Same author

DNA fragmentation of equine cumulus cells from Cumulus-Oocyte complexes submitted to vitrification and its relationship to the developmental competence of the oocyte.

Reproduction in domestic animals = Zuchthygiene·2022
Same author

Bicarbonate-Triggered In Vitro Capacitation of Boar Spermatozoa Conveys an Increased Relative Abundance of the Canonical Transient Receptor Potential Cation (TRPC) Channels 3, 4, 6 and 7 and of CatSper-γ Subunit mRNA Transcripts.

Animals : an open access journal from MDPI·2022
Same author

Hormonal Management for the Induction of Luteolysis and Ovulation in Andalusian Jennies: Effect on Reproductive Performance, Embryo Quality and Recovery Rate.

Animals : an open access journal from MDPI·2022
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Oct 23, 2025

Minimally Invasive Embryo Transfer and Embryo Vitrification at the Optimal Embryo Stage in Rabbit Model
07:02

Minimally Invasive Embryo Transfer and Embryo Vitrification at the Optimal Embryo Stage in Rabbit Model

Published on: May 16, 2019

12.9K

Recent advances in donkey sperm vitrification.

Manuel Hidalgo1

  • 1Veterinary Reproduction Group, Faculty of Veterinary Medicine, Department of Animal Medicine and Surgery, University of Cordoba, Cordoba, Spain.

Reproduction in Domestic Animals = Zuchthygiene
|August 21, 2021
PubMed
Summary
This summary is machine-generated.

Sperm vitrification offers a promising alternative to conventional freezing for preserving donkey semen. This method, using non-permeable cryoprotectant agents, shows comparable or improved sperm quality and reproductive outcomes in donkeys.

Keywords:
cryopreservationfertilityjacksemen

More Related Videos

Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts
09:35

Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts

Published on: March 2, 2017

12.1K
Spatula Montevideo Device for the Vitrification of Mammalian Embryos
07:16

Spatula Montevideo Device for the Vitrification of Mammalian Embryos

Published on: June 6, 2025

579

Related Experiment Videos

Last Updated: Oct 23, 2025

Minimally Invasive Embryo Transfer and Embryo Vitrification at the Optimal Embryo Stage in Rabbit Model
07:02

Minimally Invasive Embryo Transfer and Embryo Vitrification at the Optimal Embryo Stage in Rabbit Model

Published on: May 16, 2019

12.9K
Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts
09:35

Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts

Published on: March 2, 2017

12.1K
Spatula Montevideo Device for the Vitrification of Mammalian Embryos
07:16

Spatula Montevideo Device for the Vitrification of Mammalian Embryos

Published on: June 6, 2025

579

Area of Science:

  • Veterinary Reproduction
  • Cryobiology
  • Conservation Biology

Background:

  • Artificial insemination (AI) with cryopreserved semen is crucial for endangered species conservation, including European donkey breeds.
  • Sperm vitrification, a rapid cooling method using non-permeable cryoprotectant agents (CPAs), presents an alternative to conventional semen freezing.
  • Previous studies in donkeys explored vitrification using spheres and optimized protocols in 0.25-ml straws.

Purpose of the Study:

  • To evaluate sperm vitrification as an alternative to conventional freezing for artificial insemination in donkeys.
  • To assess the impact of different vitrification volumes and warming methods on sperm quality and reproductive efficiency.
  • To compare the efficacy of vitrified sperm with conventionally frozen semen in terms of motility, membrane integrity, and pregnancy rates.

Main Methods:

  • Sperm vitrification was initially performed in spheres using sucrose and bovine serum albumin as non-permeable CPAs.
  • An optimized aseptic protocol utilized 0.25-ml straws with outer covers, vitrifying up to 160 µl at 300 million sperm/ml.
  • A protocol for 0.5-ml straws was tested but yielded poor results; a new warming method for 0.25-ml straws was also evaluated.

Main Results:

  • Vitrification in spheres with sucrose/BSA yielded better sperm parameters post-warming than glycerol-based extenders.
  • Optimized vitrification in 0.25-ml straws showed similar total motility, progressive motility, and plasma membrane integrity compared to conventional freezing.
  • The new warming method for 0.25-ml straws resulted in faster resolution of uterine inflammation and higher pregnancy rates (22%) versus frozen semen (10%), though not statistically significant.

Conclusions:

  • Sperm vitrification is a viable alternative to conventional semen freezing for artificial insemination in jennies.
  • Optimized protocols using 0.25-ml straws and specific warming techniques enhance the potential of sperm vitrification in donkeys.
  • Further research may refine vitrification protocols for larger volumes and improve pregnancy rates for effective conservation of European donkey breeds.