Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Puncture-Needle-Integrated Optical Fibers and NIR-II-Activated Thermosensitive Hydrogels for Deep-Seated Tumor Therapy.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Two-Photon Cerebrovascular and Thrombus Fluorescence Imaging with Micrometer-Scale Penetration Depth Using Record-Low-Dose Aggregation-Induced Emission Nanoprobes.

ACS nano·2025
Same author

Multiplexed lateral flow immunoassays using high photostability gap-enhanced Raman nanotags: Highly sensitive, rapid, efficient and portable point-of-care tests.

Biosensors & bioelectronics·2025
Same author

Fast In Vivo Two-Photon Fluorescence Imaging via Lateral and Axial Resolution Restoration With Self-Supervised Learning.

Journal of biophotonics·2025
Same author

Single-Injection Composite Tracer Achieves Intraoperative Dual-Tracing and Precise Localization of Sentinel Lymph Nodes.

ACS applied materials & interfaces·2025
Same author

General Post-Regulation Strategy of AIEgens' Photophysical Properties for Intravital Two-Photon Fluorescence Imaging.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2024

Related Experiment Video

Updated: Oct 23, 2025

Surface-enhanced Resonance Raman Scattering Nanoprobe Ratiometry for Detecting Microscopic Ovarian Cancer via Folate Receptor Targeting
07:54

Surface-enhanced Resonance Raman Scattering Nanoprobe Ratiometry for Detecting Microscopic Ovarian Cancer via Folate Receptor Targeting

Published on: March 25, 2019

8.4K

Ratiometric Raman nanotags enable intraoperative detection of metastatic sentinel lymph node.

Zhouzhou Bao1, Binge Deng2, Yuqing Zhang3

  • 1Department of Obstetrics and Gynecology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 200127, Shanghai, PR China; Shanghai Key Laboratory of Gynecologic Oncology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 200127, Shanghai, PR China; State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 200127, Shanghai, PR China.

Biomaterials
|August 21, 2021
PubMed
Summary

A new ratiometric Raman dual-nanotag strategy enables accurate, intraoperative diagnosis of metastatic sentinel lymph nodes (SLNs). This imaging technique offers a promising alternative to traditional histopathology for guiding cancer surgery.

Keywords:
Lymph nodeNanoprobeRaman imagingRatiometricSurface enhanced Raman scattering

More Related Videos

Monitoring of Nanodrug Accumulation in Murine Breast Cancer Metastases
09:48

Monitoring of Nanodrug Accumulation in Murine Breast Cancer Metastases

Published on: August 23, 2024

564
Multispectral Real-time Fluorescence Imaging for Intraoperative Detection of the Sentinel Lymph Node in Gynecologic Oncology
06:37

Multispectral Real-time Fluorescence Imaging for Intraoperative Detection of the Sentinel Lymph Node in Gynecologic Oncology

Published on: October 20, 2010

23.5K

Related Experiment Videos

Last Updated: Oct 23, 2025

Surface-enhanced Resonance Raman Scattering Nanoprobe Ratiometry for Detecting Microscopic Ovarian Cancer via Folate Receptor Targeting
07:54

Surface-enhanced Resonance Raman Scattering Nanoprobe Ratiometry for Detecting Microscopic Ovarian Cancer via Folate Receptor Targeting

Published on: March 25, 2019

8.4K
Monitoring of Nanodrug Accumulation in Murine Breast Cancer Metastases
09:48

Monitoring of Nanodrug Accumulation in Murine Breast Cancer Metastases

Published on: August 23, 2024

564
Multispectral Real-time Fluorescence Imaging for Intraoperative Detection of the Sentinel Lymph Node in Gynecologic Oncology
06:37

Multispectral Real-time Fluorescence Imaging for Intraoperative Detection of the Sentinel Lymph Node in Gynecologic Oncology

Published on: October 20, 2010

23.5K

Area of Science:

  • Nanotechnology
  • Medical Imaging
  • Oncology

Background:

  • Sentinel lymph node (SLN) biopsy is crucial for cancer staging and surgical planning.
  • Current methods for diagnosing SLN metastasis during surgery lack accuracy and speed.

Purpose of the Study:

  • To develop a novel, rapid, and accurate intraoperative method for diagnosing metastatic SLNs.
  • To introduce the "Ratiometric Raman dual-nanotag strategy" for enhanced SLN imaging.

Main Methods:

  • Utilized folic acid functionalized targeted and nontargeted gap-enhanced Raman tags (FA-GERTs and Nt-GERTs).
  • Employed Raman imaging combined with classical least square data processing.
  • Implemented a ratiometric signal readout with built-in self-calibration for signal correction.

Main Results:

  • Achieved visualization and prompt intraoperative diagnosis of metastatic SLNs.
  • Demonstrated a high diagnostic accuracy of 87.5% using the FA-GERTs/Nt-GERTs ratio.
  • The developed strategy showed potential to surpass current histopathological assessments.

Conclusions:

  • The Ratiometric Raman dual-nanotag strategy provides accurate and rapid intraoperative diagnosis of SLN metastasis.
  • This technique holds promise for improving surgical guidance in cancer treatment.
  • Further development could enhance its clinical applicability in oncology.