Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Diversity of Antigen Receptors01:28

Diversity of Antigen Receptors

990
Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
990
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

4.3K
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.3K
Cancer-Critical Genes I: Proto-oncogenes01:33

Cancer-Critical Genes I: Proto-oncogenes

9.4K
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
9.4K
Abnormal Proliferation02:23

Abnormal Proliferation

4.7K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.7K
Targeted Cancer Therapies02:57

Targeted Cancer Therapies

8.0K
The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
8.0K
Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

5.6K
Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...
5.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

All-arthroscopic release in treating knee stiffness after anterior cruciate ligament reconstruction.

Annals of joint·2026
Same author

The evolution and frontiers of global pediatric glucocorticoids research: an in-depth analysis based on 28 years of bibliometrics.

Naunyn-Schmiedeberg's archives of pharmacology·2026
Same author

Correction: A mammalian, glutaminase-free asparaginase enhances venetoclax activity in preclinical AML models with chromosome 7 deletion.

Frontiers in oncology·2026
Same author

Two-step pyrolysis enabled by bentonite catalysis for the co-production of bioactive humic acid and functional carbon-clay composites.

Environmental research·2026
Same author

Minimally Invasive Keyhole Interlaminar Detethering for Symptomatic Fatty Filum Terminale in Pediatric Patients: An Operative Technique.

World neurosurgery·2026
Same author

Clinical Efficacy Evaluation of Percutaneous Endoscopic Interlaminar Decompression Under Local Anesthesia for the Treatment of Degenerative Lumbar Spinal Stenosis in Elderly Patients with Medical Comorbidities: A Multicenter Retrospective Study.

World neurosurgery·2026
Same journal

Deciphering the Neuroautophagic Interactome: Molecular Circuits Linking Selective Autophagy to Neuropathological Cascades in Neurological Disorders.

International journal of biological sciences·2026
Same journal

Targeting THOC2-Mediated mRNA Export Induces PARP Inhibitor Vulnerability in DNA Repair-Competent Hepatocellular Carcinoma.

International journal of biological sciences·2026
Same journal

Targeting IL-1α Sensitizes HNSCC to PDT by Reversing Hypoxia and NF-κB-Driven Oxidative Stress Resistance.

International journal of biological sciences·2026
Same journal

Nicotinamide riboside reduces glial inflammation and boosts mitochondrial function.

International journal of biological sciences·2026
Same journal

Spatial and single-cell transcriptomics reveal a HIF-1α/NF-κB-driven hypoxia-induced senescence axis in BPH epithelium.

International journal of biological sciences·2026
Same journal

Sleep Deprivation Promotes Colorectal Cancer in Apc<sup>Min/+</sup> Mice Associated with Modulation of Gut Microbiota and Related Metabolites.

International journal of biological sciences·2026
See all related articles

Related Experiment Video

Updated: Oct 23, 2025

Identifying the Effects of BRCA1 Mutations on Homologous Recombination using Cells that Express Endogenous Wild-type BRCA1
08:53

Identifying the Effects of BRCA1 Mutations on Homologous Recombination using Cells that Express Endogenous Wild-type BRCA1

Published on: February 17, 2011

14.9K

BRCA1 Antibodies Matter.

Jing Yang1, Leilei Qi1, Huai-Chin Chiang2

  • 1Department of Anatomy & Cell Biology, School of Medicine & Health Sciences, The George Washington University, Washington, DC, USA.

International Journal of Biological Sciences
|August 23, 2021
PubMed
Summary
This summary is machine-generated.

This study validates commercial BRCA1 antibodies for reliable research. Specific antibodies for human and mouse BRCA1 were identified, improving data reproducibility and understanding of BRCA1 functions.

Keywords:
Antibody validationBRCA1Chromatin ImmunoprecipitationImmunofluorescenceImmunoprecipitationWestern blot

More Related Videos

Silencing of BRCA2 to Identify Novel BRCA2-regulated Biological Functions in Cultured Human Cells
09:24

Silencing of BRCA2 to Identify Novel BRCA2-regulated Biological Functions in Cultured Human Cells

Published on: August 12, 2015

9.3K
Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors
09:22

Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors

Published on: February 28, 2021

5.7K

Related Experiment Videos

Last Updated: Oct 23, 2025

Identifying the Effects of BRCA1 Mutations on Homologous Recombination using Cells that Express Endogenous Wild-type BRCA1
08:53

Identifying the Effects of BRCA1 Mutations on Homologous Recombination using Cells that Express Endogenous Wild-type BRCA1

Published on: February 17, 2011

14.9K
Silencing of BRCA2 to Identify Novel BRCA2-regulated Biological Functions in Cultured Human Cells
09:24

Silencing of BRCA2 to Identify Novel BRCA2-regulated Biological Functions in Cultured Human Cells

Published on: August 12, 2015

9.3K
Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors
09:22

Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors

Published on: February 28, 2021

5.7K

Area of Science:

  • Molecular Biology
  • Genetics
  • Cancer Research

Background:

  • Breast cancer susceptibility gene 1 (BRCA1) is a crucial tumor suppressor frequently mutated in hereditary breast and ovarian cancers.
  • BRCA1 plays vital roles in DNA repair, cell cycle control, and other cellular processes.
  • Many commercially available BRCA1 antibodies lack rigorous validation, leading to unreliable research data.

Purpose of the Study:

  • To rigorously validate commercially available anti-BRCA1 antibodies for specificity and performance.
  • To identify reliable antibodies for detecting both human and mouse BRCA1 protein.
  • To aid researchers in overcoming challenges with antibody specificity and reproducibility in BRCA1 studies.

Main Methods:

  • Validation of anti-BRCA1 antibodies using Western blot (WB), immunoprecipitation (IP), IP-mass spectrometry (IP-MS), chromatin immunoprecipitation (ChIP), and immunofluorescence (IF).
  • Assessment of antibody specificity in detecting mouse BRCA1 using tissues and cells from Brca1 wild-type and knockout mice.
  • Utilized proper controls throughout the validation process.

Main Results:

  • Identified specific and high-performing antibodies for human BRCA1: Ab1, D-9, and 07-434.
  • Identified specific and high-performing antibodies for mouse BRCA1: 287.17, 440621, and BR-64.
  • Demonstrated the utility of these validated antibodies across multiple experimental applications.

Conclusions:

  • Provides a curated list of validated anti-BRCA1 antibodies to enhance research reproducibility.
  • Facilitates more accurate studies on BRCA1 functions in cellular and tissue contexts.
  • Addresses a critical need for reliable tools in cancer susceptibility gene research.