Related Experiment Video

Updated: Oct 23, 2025

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
09:34

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Published on: April 4, 2018

34.2K

Comprehensive Bioinformatics Analysis Identifies POLR2I as a Key Gene in the Pathogenesis of Hypertensive Nephropathy

Shilong You1, Jiaqi Xu1, Boquan Wu1

  • 1Department of Cardiology, The First Hospital of China Medical University, Shenyang, China.

Frontiers in Genetics
|August 23, 2021
PubMed

Insights

Hypertensive nephropathy (HN) pathogenesis is unclear. Researchers identified POLR2I as a key gene, finding its increased expression correlates with kidney function in HN patients.

Area of Science:

  • Nephrology
  • Genomics
  • Molecular Biology

Background:

  • Hypertensive nephropathy (HN) is a leading cause of end-stage renal disease.
  • The underlying molecular mechanisms of HN remain poorly understood.
  • Effective treatments for HN are urgently needed.

Purpose of the Study:

  • To identify key genes involved in the pathogenesis of hypertensive nephropathy.
  • To elucidate the molecular mechanisms contributing to HN.
  • To find potential therapeutic targets for HN.

Main Methods:

  • Gene expression profiles from HN and normal tissues were analyzed.
  • Weighted gene co-expression network analysis and differential gene expression analysis were performed.
  • Protein-protein interaction networks were constructed to identify hub genes, including POLR2I, which were validated in independent datasets.

Main Results:

  • A total of 229 differentially co-expressed genes were identified.
  • POLR2I was identified as a crucial hub gene in HN pathogenesis.
  • POLR2I expression was upregulated in HN and positively correlated with renal function, with in vivo validation.

Conclusions:

  • POLR2I is a key gene implicated in the pathogenesis of hypertensive nephropathy.
  • Upregulation of POLR2I is associated with disease progression and renal function in HN.
  • This study provides novel insights into HN's molecular mechanisms and identifies POLR2I as a potential therapeutic target.

Keywords:
POLR2Idifferentially expressed genes (DEGs)hypertensive nephropathy (HN)key genepathogenesisweighted gene co-expression network analysis (WGCNA)

More Related Videos

A Modified Two Kidney One Clip Mouse Model of Renin Regulation in Renal Artery Stenosis08:21

A Modified Two Kidney One Clip Mouse Model of Renin Regulation in Renal Artery Stenosis

Published on: October 26, 2020

5.1K
Investigating the Pathogenesis of MYH7 Mutation Gly823Glu in Familial Hypertrophic Cardiomyopathy using a Mouse Model03:45

Investigating the Pathogenesis of MYH7 Mutation Gly823Glu in Familial Hypertrophic Cardiomyopathy using a Mouse Model

Published on: August 8, 2022

3.8K

Related Concept Videos

Hypertension II: Pathophysiology01:29

Hypertension II: Pathophysiology

Hypertension is a chronic condition in which the blood's force against artery walls is excessively high, posing risks such as heart disease. The condition's underlying mechanisms involve complex interactions among the cardiovascular, kidney, and autonomic nervous systems.Renin-Angiotensin-Aldosterone System (RAAS): This system significantly influences blood pressure regulation. When blood pressure decreases, the kidneys secrete renin. This enzyme transforms angiotensinogen, a plasma protein,...
189
Hypertension and Regulation of Blood Pressure01:18

Hypertension and Regulation of Blood Pressure

Hypertension, the most common cardiovascular disease, is diagnosed through repeated measurements of elevated blood pressure. Its risks, including damage to the kidney, heart, and brain, are directly proportional to blood pressure levels. Starting from 115/75 mm Hg, the risk of cardiovascular disease doubles with each increment of 20/10 mm Hg. The diagnosis relies on blood pressure measurements, not on patient symptoms, as hypertension is often asymptomatic until end-organ damage is imminent or...
3.4K
Pulmonary Hypertension: Classification and Pathogenesis01:30

Pulmonary Hypertension: Classification and Pathogenesis

Pulmonary hypertension (PH) is a severe health condition in which the mean pulmonary arterial pressure increases to 25 mmHg or more, even when the body is at rest. This high pressure in the blood vessels that transport blood from the heart to the lungs can cause various symptoms, including shortness of breath, can lead to right heart failure, and significantly affect the overall quality of life.
There are various classifications for PH, each relating to different underlying causes and also...
344
Blood Studies for Cardiovascular System II: CRP, Hcy, and Cardiac Natriuretic Peptide Markers01:19

Blood Studies for Cardiovascular System II: CRP, Hcy, and Cardiac Natriuretic Peptide Markers

Cardiac biomarkers are critical in diagnosing, prognosing, and managing cardiovascular diseases. Routine measurement of specific biomarkers such as B-type natriuretic peptide (BNP), C-reactive protein (CRP), and homocysteine (Hcy) is common practice in clinical settings to evaluate heart function and predict cardiovascular events.
These markers indicate stress or strain on the heart muscle:
Natriuretic Peptides (BNP)
Cardiac myocytes produce these hormones in response to ventricular stretching...
267
Antihypertensive Drugs: Angiotensin-Converting Enzyme Inhibitors01:30

Antihypertensive Drugs: Angiotensin-Converting Enzyme Inhibitors

Angiotensin-converting enzyme (ACE), a vital component of the renin-angiotensin-aldosterone system, is abundant in lung endothelial cells. ACE converts the inactive decapeptide, angiotensin I, into the active octapeptide, angiotensin II. This potent vasoconstrictor narrows blood vessels, increasing resistance to blood flow and elevating blood pressure. Angiotensin II also stimulates aldosterone production, encouraging kidney cells to reabsorb more sodium and water from urine, thereby increasing...
1.8K