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TMEM100 Modulates TGF-β Signaling Pathway to Inhibit Colorectal Cancer Progression
Huixia Li1, Chuan Cheng1, Weibo You1
1Department of Colorectal and Anal Surgery, Lishui Municipal Central Hospital, Affiliated Lishui Hospital of Zhejiang University, Lishui 323000, China.
Objectives:
This study investigated the functional mechanism of transmembrane protein 100 (TMEM100) as a tumor inhibitor gene in CRC cells and offered a reference for the treatment of CRC.
Methods:
The mRNA expression data of CRC were acquired from the TCGA database to mine differentially expressed mRNAs. The role of TMEM100 in the progression of CRC cells was evaluated by MTT, colony formation, scratch healing, and Transwell assays. The influence of TMEM100 on the TGF-β signaling pathway was detected by western blot.
Results:
TMEM100 was markedly lowly expressed in CRC. CRC cell growth was significantly suppressed by overexpressing TMEM100 but noticeably facilitated by silencing TMEM100. Overexpression of TMEM100 inhibited the activation of the TGF-β signaling pathway, thus inhibiting malignant progression of CRC.
Conclusion:
TMEM100 is lowly expressed in CRC, which can suppress CRC cell growth by regulating the TGF-β signaling pathway.
Insights
Transmembrane protein 100 (TMEM100) acts as a tumor suppressor in colorectal cancer (CRC). Low TMEM100 expression promotes CRC cell growth by activating the TGF-β signaling pathway.
Area of Science:
- Oncology
- Molecular Biology
- Cancer Genetics
Background:
- Colorectal cancer (CRC) remains a significant global health challenge.
- Understanding the molecular mechanisms underlying CRC progression is crucial for developing effective therapies.
- The role of transmembrane protein 100 (TMEM100) in CRC has not been fully elucidated.
Purpose of the Study:
- To investigate the functional mechanism of TMEM100 as a tumor suppressor in colorectal cancer (CRC) cells.
- To explore the potential of TMEM100 as a therapeutic target for CRC treatment.
- To elucidate the relationship between TMEM100 and the TGF-β signaling pathway in CRC.
Main Methods:
- Utilized The Cancer Genome Atlas (TCGA) database to analyze mRNA expression data in CRC.
- Employed MTT, colony formation, scratch healing, and Transwell assays to assess CRC cell progression.
- Investigated the impact of TMEM100 on the TGF-β signaling pathway using western blot analysis.
Main Results:
- TMEM100 exhibited significantly low expression in colorectal cancer tissues.
- Overexpression of TMEM100 suppressed CRC cell growth, while its silencing facilitated growth.
- TMEM100 overexpression inhibited the activation of the TGF-β signaling pathway, thereby hindering malignant progression.
Conclusions:
- TMEM100 functions as a tumor suppressor gene in colorectal cancer.
- Low TMEM100 expression is associated with enhanced CRC cell proliferation.
- TMEM100 suppresses CRC growth by negatively regulating the TGF-β signaling pathway.
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