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On chromosomal DNA modifications by chemical and physical treatment of C-bands
L Stuppia1, G Calabrese, P Guanciali Franchi
1Centro di Citomorfologia Umana C.N.R., Chieti, Italy.
Abstract:
Experiments were performed on fixed metaphase chromosomes using standard techniques for revealing paracentromeric heterochromatin (C bands) followed by staining with acridine orange with the aim of studying C-banding mechanism. Data obtained suggest that the specific resistance to the chemical-physical treatments of the heterochromatic areas is a consequence of the particular structural conditions that the C-positive material shows only after its early renaturation.