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Related Experiment Video

Updated: Oct 23, 2025

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

Published on: June 16, 2017

Exosomal DNMT1 mRNA transcript is elevated in acute lymphoblastic leukemia which might reprograms leukemia

Shabirul Haque¹, Sarah R Vaiselbuh²

¹The Feinstein Institute for Medical Research, Northwell Health, 350 Community Drive, Manhasset, NY 11030, USA; Department of Pediatrics, Staten Island University Hospital, Northwell Health, 475 Seaview Ave, Staten Island, NY 10305, USA.

Cancer Genetics

|August 24, 2021

Summary

DNA-methyltransferase 1 (DNMT1) mRNA is elevated in leukemia exosomes and can transfer to target cells. This transfer may reprogram cells, influencing leukemia progression and epigenetic changes.

Keywords:

Acute lymphocytic leukemia Epigenetics, DNMT1 Exosomes

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Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

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Author Spotlight: Analyzing Bone Marrow Microenvironment in Murine Hematological Malignancies

Author Spotlight: Analyzing Bone Marrow Microenvironment in Murine Hematological Malignancies

Published on: November 10, 2023

Area of Science:

Biochemistry
Molecular Biology
Cancer Research

Background:

DNA-methyltransferase 1 (DNMT1) is crucial for embryonic development.
Aberrant DNMT1 expression drives tumor and leukemia progression via epigenetic alterations.
Exosomes play a role in intercellular communication and disease progression.

Purpose of the Study:

To investigate DNMT1 mRNA levels in exosomes derived from leukemia.
To determine if exosomal DNMT1 mRNA can be transferred to target cells.
To explore the potential role of exosomal DNMT1 in leukemia reprogramming.

Main Methods:

Quantitative analysis of DNMT1 mRNA in exosomes (Exo-PALL, Exo-HD, Exo-CM).
Co-culture experiments using leukemia B cells as target cells.
Detection of exosomal DNMT1 mRNA transfer into target cells.

Main Results:

DNMT1 mRNA transcripts were significantly elevated in Exo-PALL compared to Exo-HD.
Heightened DNMT1 mRNA levels were confirmed in exosomes from leukemia cell lines (Exo-CM).
Transfer of exosomal DNMT1 mRNA into target leukemia B cells was observed.

Conclusions:

Exosomes from leukemia cells carry elevated levels of DNMT1 mRNA.
Exosomal DNMT1 mRNA can be transferred to recipient leukemia cells.
This transfer mechanism may contribute to leukemia progression by reprogramming cellular epigenetics.