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Tutorial: using nanoneedles for intracellular delivery.

Ciro Chiappini1,2, Yaping Chen3,4, Stella Aslanoglou3,4,5

  • 1Centre for Craniofacial and Regenerative Biology, King's College London, London, UK. ciro.chiappini@kcl.ac.uk.

Nature Protocols
|August 24, 2021
PubMed
Summary
This summary is machine-generated.

Nanoneedles offer a precise method for intracellular delivery of therapeutics, overcoming biological barriers. This tutorial guides researchers in adopting nanoneedle technology for advanced drug delivery and cell therapy applications.

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Area of Science:

  • Biotechnology
  • Nanotechnology
  • Cell Biology

Background:

  • Intracellular delivery of therapeutics like nucleic acids, proteins, and nanoparticles faces significant biological barriers.
  • Efficient intracellular delivery is essential for the clinical success of gene and cell therapies.
  • Nanoneedle technology provides a promising solution for direct and non-destructive intracellular access.

Purpose of the Study:

  • To provide researchers with essential knowledge for developing a nanoinjection workflow.
  • To facilitate the adoption of nanoneedle technology for intracellular drug delivery.
  • To overcome the complexity and interdisciplinary nature hindering nanoneedle platform implementation.

Main Methods:

  • Discussion on selecting appropriate nanoneedle devices.
  • Exploration of various cargo loading strategies for therapeutic payloads.
  • Guidance on interfacing nanoneedles with biological systems.
  • Summary of bioassays for evaluating intracellular delivery efficacy.

Main Results:

  • Nanoneedles enable efficient and non-destructive intracellular delivery of cell-impermeant payloads.
  • The technology allows precise manipulation at the cell interface for targeted delivery.
  • This tutorial aims to demystify nanoneedle implementation for a broader research audience.

Conclusions:

  • Nanoneedle technology represents a significant advancement in intracellular drug delivery.
  • Standardized workflows and accessible knowledge are crucial for widespread adoption.
  • This tutorial equips researchers to leverage nanoneedles for enhanced therapeutic development.