Effect of induced sublethal intoxication with neonicotinoid insecticides on Egyptian toads (Sclerophrys regularis)

The toxicity role of insecticides affecting nontarget vertebrate of wildlife population has become essential subject to focus on. In this vein, the current study aimed to illustrate some biochemical and histopathological alterations induced by two neonicotinoids in Egyptian toads. Forty-five toads were collected and divided equally into three groups (15 toads/group): control group, thiamethoxam group, and acetamiprid group. Both treatment groups received thiamethoxam and acetamiprid (30 and 40 mg/L, respectively) four times within 12 days for induction of sublethal toxicity. Blood and liver tissue samples were collected. Both insecticides cause the same changes, but acetamiprid group exhibited a pronounced significant (P ≥ 0.001) effect than thiamethoxam group on increasing serum lipid profile, ALT, and AST. Moreover, acetamiprid showed a significant (P ≥ 0.001) decrease in hepatic total protein, GSH, and SOD and increase in MDA levels in comparison with thiamethoxam and control groups, respectively. The histopathological hepatic examination showed markable alterations in hepatic architecture in treatment groups that was distinct in acetamiprid group. Finally, our findings illustrate the indirect effect of neonicotinoids on toads and may realize their life-threatening factors. Graphical Abstract.