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[The mouse B16 melanoma in a 3-dimensional culture]
M C De Pauw-Gillet1, Y Christiane, J M Foidart
1Service d'Histologie et de Cytologie, Université de Liège, Belgique.
Bulletin De L'Association Des Anatomistes
|September 1, 1986
Abstract:
Multicellular spheroids composed of mouse B16 melanoma cells have been prepared in non agitated culture on solid agar. Cells present frequent contacts and an extracellular matrix appears during cultivation (up to 2 months in agitated suspension); it contains laminin, fibronectin and collagen I, III and IV. Cellular differentiation takes place inside these spheroids (melanogenesis progressively increases), but a relatively intense cell proliferation rate is also maintained.