Detection of a novel species A, DS-1-like, G4P[6] rotavirus strain from a Brazilian child with gastroenteritis

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Renato da Silva Bandeira1, Lizandra Caroline Dos Santos Souto2, Layse Costa de Souza2

  • 1Brazilian Ministry of Health, Health Surveillance Secretariat, Evandro Chagas Institute, Postgraduate program in virology, Virology Section, Levilândia, Ananindeua-Pará, Brazil.

Journal of Medical Virology
|August 24, 2021
PubMed

Insights

A novel Rotavirus A (RVA) G4P[6] strain was identified, likely resulting from zoonotic transmission and reassortment between human and animal strains. This finding highlights potential new pathways for RVA evolution and spread.

Area of Science:

  • Virology
  • Molecular Biology
  • Public Health

Background:

  • Rotavirus A (RVA) causes severe gastroenteritis in young children globally.
  • Genome reassortment between human and animal strains drives RVA genetic diversity.
  • RVA strains are classified into three genotype constellations: Wa-like (genogroup I), DS-1-like (genogroup II), and AU-like (genogroup III).

Purpose of the Study:

  • To report the detection and characterization of an unusual RVA G4P[6] strain.
  • To investigate the potential zoonotic origin and reassortment events of this novel strain.
  • To highlight the evolutionary dynamics of circulating RVA strains.

Main Methods:

  • Detection and isolation of RVA strain HSE005.
  • Molecular characterization including nucleotide sequencing.
  • Phylogenetic analysis to determine genotype and genogroup.
  • Identification of reassortment events in specific viral genes.

Main Results:

  • An unusual RVA G4P[6] strain (HSE005) was detected.
  • The strain belonged to genogroup II and genotype G4P[6].
  • Evidence of reassortment involving the VP3 and NSP4 genes, with potential zoonotic origins, was found.

Conclusions:

  • A novel G4P[6] RVA strain has been identified.
  • This strain may represent a significant zoonotic transmission event involving reassortment.
  • The findings underscore the importance of monitoring RVA evolution and interspecies transmission.

Keywords:
G4P[6]Rotavirus Agenogroup IInew strainzoonotic

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