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Updated: Oct 23, 2025

A Multimodal Imaging Approach Based on Micro-CT and Fluorescence Molecular Tomography for Longitudinal Assessment of Bleomycin-Induced Lung Fibrosis in Mice

A Multimodal Imaging Approach Based on Micro-CT and Fluorescence Molecular Tomography for Longitudinal Assessment of Bleomycin-Induced Lung Fibrosis in Mice

Published on: April 13, 2018

Comprehensive dataset to assess morphological changes subsequent to bleomycin exposure.

Thea Golden¹, Alexa Murray², Alessandro Venosa³

¹Center for Research on Reproduction and Women's Health, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 18015, USA.

|August 25, 2021

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Intratracheal bleomycin induces patchy lung injury. A new multi-factorial method using ImageJ quantifies morphological changes for robust analysis of this heterogeneous pulmonary damage.

Area of Science:

Pulmonary Pathology
Toxicology
Medical Imaging Analysis

Background:

Intratracheal bleomycin administration is a common model for inducing lung injury, inflammation, and fibrosis.
The patchy, heterogeneous nature of bleomycin-induced lung injury presents significant challenges for accurate histological assessment and quantification.
Standardized methods for quantifying lung injury in this model are lacking, hindering comparative studies.

Purpose of the Study:

To develop and validate a multi-factorial approach for assessing morphological changes in bleomycin-induced lung injury.
To provide a standardized and robust method for quantifying heterogeneous lung damage.
To improve the analysis of lung injury in preclinical research models.

Main Methods:

Lungs from mice treated with intratracheal bleomycin were inflation-fixed and processed for histological analysis.

Keywords:

Acute lung injury Bleomycin injury Experimental lung Inflammation Fibrosis Histological scoring

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Last Updated: Oct 23, 2025

A Multimodal Imaging Approach Based on Micro-CT and Fluorescence Molecular Tomography for Longitudinal Assessment of Bleomycin-Induced Lung Fibrosis in Mice

A Multimodal Imaging Approach Based on Micro-CT and Fluorescence Molecular Tomography for Longitudinal Assessment of Bleomycin-Induced Lung Fibrosis in Mice

Published on: April 13, 2018

Oropharyngeal Administration of Bleomycin in the Murine Model of Pulmonary Fibrosis

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Published on: May 9, 2025

Unilateral Lung Volume Analysis Using Micro-CT for Enhanced Assessment of Pulmonary Fibrosis in Preclinical Models

Unilateral Lung Volume Analysis Using Micro-CT for Enhanced Assessment of Pulmonary Fibrosis in Preclinical Models

Published on: June 20, 2025

Whole slide images were acquired, and ten random 400x fields of view were analyzed per lung.

ImageJ software was utilized to measure alveolar wall width, count nuclei, and quantify airspace dimensions.

Main Results:

Morphological alterations consistent with lung injury were identified in bleomycin-treated mice.
The multi-factorial approach enabled quantitative assessment of alveolar wall thickening, cellularity, and airspace enlargement.
The method demonstrated robustness in characterizing the heterogeneous injury patterns.

Conclusions:

A combination of histological analysis and ImageJ quantification provides a robust measure of lung morphology after bleomycin injury.
This standardized approach enhances the ability to analyze and compare heterogeneous pulmonary damage in research settings.
The proposed method facilitates more accurate evaluation of therapeutic interventions targeting lung injury and fibrosis.