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Related Experiment Videos

The chicken progesterone receptor: sequence, expression and functional analysis.

H Gronemeyer1, B Turcotte, C Quirin-Stricker

  • 1Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Faculté de Médicine, Strasbourg, France.

The EMBO Journal
|December 20, 1987
PubMed
Summary

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The chicken progesterone receptor (cPR) mRNA sequence was determined, revealing cloned cPR functions identically to the natural receptor. Form A is likely generated via cell-specific internal translation initiation or proteolysis.

Area of Science:

  • Molecular Biology
  • Endocrinology
  • Genetics

Background:

  • The progesterone receptor (PR) is a key regulator of reproductive processes.
  • Understanding PR structure and function is crucial for reproductive health research.
  • Chicken progesterone receptor (cPR) serves as a model for steroid hormone receptors.

Purpose of the Study:

  • To determine the complete mRNA sequence of the chicken progesterone receptor (cPR).
  • To characterize the functional properties of the cloned cPR.
  • To elucidate the mechanisms generating different cPR forms and their functional domains.

Main Methods:

  • mRNA sequencing and cDNA cloning of cPR.
  • In vivo and in vitro expression of cPR in various cell lines (HeLa, Cos-1).

Related Experiment Videos

  • Immunoblotting, photoaffinity labeling, hormone binding assays, and nuclear binding studies.
  • Analysis of cPR deletion mutants and transcriptional activation assays using MMTV-LTR.

    • Main Results:

    • Cloned cPR expression yielded proteins indistinguishable from natural cPR forms B and A in size, hormone binding, and nuclear localization.
    • Form A appears to be generated by oviduct cell-specific internal translation initiation or proteolysis near methionine-128.
    • Hormone binding domain (region E) and nuclear association domain (region C) were identified. Region C is critical for MMTV-LTR transcriptional activation.

    Conclusions:

    • The cloned cPR accurately reflects the properties of the native receptor.
    • Specific cellular mechanisms generate distinct cPR forms with functional implications.
    • Key functional domains of cPR, including hormone binding and transcriptional activation, have been mapped.