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[USF1 Suppresses Expression of Fibrillar Type I, II, and III Collagen and pNP Adamts-3 in Osteosarcoma Cells]

M Alper1,2, T Aydemir3, F Köçkar4

  • 1Medicinal and Aromatic Plants, Vocational School of Technical Sciences, Aksaray University, Aksaray, Turkey.

Molekuliarnaia Biologiia
|August 25, 2021
PubMed

Insights

Transcription factor USF1 (Ulysses Factor 1) negatively regulates collagen production by decreasing expression of collagen genes and ADAMTS-3 in osteosarcoma cells.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Collagens are crucial structural proteins in human tissues.
  • Dysregulation of collagen gene expression is linked to various pathologies.
  • Understanding collagen gene regulation is vital for treating related diseases.

Purpose of the Study:

  • To investigate the regulatory role of USF1 (Ulysses Factor 1) in collagen gene expression.
  • To determine the effect of USF1 on ADAMTS-3, a key collagen-processing proteinase.
  • To elucidate the mechanism of USF1-mediated regulation in osteosarcoma cells.

Main Methods:

  • Ectopic expression of USF1 in Saos-2 and MG-63 osteoblastic cells.
  • Quantitative analysis of mRNA and protein levels for collagen types I, II, III, and ADAMTS-3.
  • Promoter activity assays (co-transfection).
  • Electrophoretic Mobility Shift Assays (EMSA) to confirm USF1 binding to the ADAMTS-3 promoter.

Main Results:

  • Ectopic USF1 expression reduced mRNA and protein levels of fibrillar collagens (types I, II, III).
  • USF1 overexpression decreased both mRNA and protein expression of ADAMTS-3.
  • USF1 was shown to bind to the ADAMTS-3 promoter region.
  • USF1 negatively regulated ADAMTS-3 promoter activity in co-transfection studies.

Conclusions:

  • USF1 acts as a negative regulator of collagen-encoding genes in osteosarcoma cells.
  • USF1 directly influences ADAMTS-3 expression by binding to its promoter.
  • Both USF1 and ADAMTS-3 are implicated in the regulation of collagen genes within the context of osteosarcoma.
Keywords:
ADAMTS-3USF1collagenosteosarcomatranscriptional regulation

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