Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Cerebellum: Anatomical Regions

Cerebellum: Anatomical Regions

The cerebellum, also known as the "little brain," is located in the posterior cranial fossa, inferior to the tentorium cerebelli and dorsal to the brainstem. It plays a significant role in motor control, coordination, and proprioception.
Cerebellar Structure
Externally, the cerebellum features a highly convoluted surface with numerous folia (narrow ridges) separated by shallow sulci (grooves). The cerebellum is divided into two hemispheres by a thin median structure known as the vermis. The...

Cerebrospinal Fluid

Cerebrospinal Fluid

Cerebrospinal fluid (CSF) is a colorless liquid that flows around the brain and the spinal cord, playing a vital role in the protection, support, and overall function of the central nervous system (CNS). CSF production, circulation, and absorption are tightly regulated processes essential for the brain and spinal cord to function properly.
CSF Production
CSF is produced mainly in the choroid plexus, a network of capillaries and ependymal cells located within the ventricular system of the brain.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Polarity-dependent modulation of sensory circuits by cerebellar tDCS: local and distant effects.

Scientific reports·2026

Same author

Consensus Paper: Models of Cerebellar Functions.

Cerebellum (London, England)·2026

Same author

Coordination of spike timing among the neurons of the cerebellum.

Research square·2026

Same author

A neural language for the cerebellum: control of behavior via competing populations of Purkinje cells.

Journal of neurophysiology·2026

Same author

Coordination of spike timing among the neurons of the cerebellum.

bioRxiv : the preprint server for biology·2025

Same author

Separating the control of moving and holding in human post-stroke arm paresis.

eLife·2025

Search research articles

Related Experiment Video

Updated: Jul 2, 2026

Cortical Source Analysis of High-Density EEG Recordings in Children

Cortical Source Analysis of High-Density EEG Recordings in Children

Published on: June 30, 2014

P-sort: an open-source software for cerebellar neurophysiology.

Ehsan Sedaghat-Nejad¹, Mohammad Amin Fakharian^1,2, Jay Pi¹

¹Laboratory for Computational Motor Control, Department of Biomedical Engineering, Johns Hopkins School of Medicine, Baltimore, Maryland.

Journal of Neurophysiology

|August 25, 2021

Summary

We developed P-sort, a novel software for analyzing cerebellar Purkinje cell (P-cell) electrophysiology. P-sort improves the identification and attribution of simple and complex spikes, outperforming existing methods.

Keywords:

Purkinje cell cerebellum open database open-source software spike sorter

More Related Videos

A Visual Guide to Sorting Electrophysiological Recordings Using 'SpikeSorter'

A Visual Guide to Sorting Electrophysiological Recordings Using 'SpikeSorter'

Published on: February 10, 2017

Simultaneous Eye Tracking and Single-Neuron Recordings in Human Epilepsy Patients

Simultaneous Eye Tracking and Single-Neuron Recordings in Human Epilepsy Patients

Published on: June 17, 2019

Related Experiment Videos

Last Updated: Jul 2, 2026

Cortical Source Analysis of High-Density EEG Recordings in Children

Cortical Source Analysis of High-Density EEG Recordings in Children

Published on: June 30, 2014

A Visual Guide to Sorting Electrophysiological Recordings Using 'SpikeSorter'

A Visual Guide to Sorting Electrophysiological Recordings Using 'SpikeSorter'

Published on: February 10, 2017

Simultaneous Eye Tracking and Single-Neuron Recordings in Human Epilepsy Patients

Simultaneous Eye Tracking and Single-Neuron Recordings in Human Epilepsy Patients

Published on: June 17, 2019

Area of Science:

Neuroscience
Computational Neuroscience

Background:

Electrophysiological data analysis from cerebellar Purkinje cells (P-cells) poses challenges for spike sorting due to waveform variability.
Complex spikes in P-cells exhibit significant waveform variations, leading to misidentification, and simple spikes may be misattributed to different P-cells.

Purpose of the Study:

To develop and validate an open-source, semiautomated software, P-sort, for accurate spike sorting of cerebellar P-cells.
To address the identification and attribution problems of simple and complex spikes in P-cell recordings.

Main Methods:

P-sort utilizes nonlinear dimensionality reduction for spike clustering, incorporating the statistical relationship between simple and complex spikes to refine clusters.
The software was tested on P-cell data recorded from three species: marmosets, macaques, and mice.

Main Results:

P-sort demonstrated improved identification of complex spikes and prevented cluster misattribution compared to expert manual curation.
Existing automatic sorters performed less effectively, particularly in identifying complex spikes.

Conclusions:

P-sort offers a significant advancement in analyzing cerebellar P-cell electrophysiological data.
The study provides valuable labeled data and statistical insights into P-cell firing patterns across three species to aid future research.