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Updated: Oct 23, 2025

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
Rapid and improved identification of drinking water bacteria using the Drinking Water Library, a dedicated MALDI-TOF
Anna Pinar-Méndez1, Sonia Fernández2, David Baquero2
1Aigües de Barcelona, Empresa Metropolitana de Gestió del Cicle Integral de l'Aigua, General Batet 1-7, Barcelona 08028, Spain; Department of Genetics, Microbiology and Statistics, University of Barcelona, Avinguda Diagonal, 643, 08028 Barcelona, Spain; The Water Research Institute, University of Barcelona, Barcelona, Spain.
A new drinking water library (DWL) for matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) significantly improves bacterial identification in water. This tool aids in the microbiological control of drinking and bottled water quality.
Area of Science:
- Microbiology
- Analytical Chemistry
- Environmental Science
Background:
- European Directives mandate specific bacterial limits for safe drinking water.
- Current methods for bacterial identification in drinking water can be time-consuming.
- Existing mass spectrometry libraries are primarily clinical, limiting their environmental application.
Purpose of the Study:
- To develop a specialized matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) library for drinking water bacteria.
- To enhance the speed and accuracy of microbiological control in water treatment facilities.
- To improve the identification of culturable bacteria in water intended for human consumption.
Main Methods:
- Creation of a dedicated drinking water library (DWL) using MALDI-TOF MS.
- Inclusion of 319 bacterial strains relevant to drinking and bottled water.
- Development of a database comprising 96 bacterial genera, with 44 novel entries.
Main Results:
- The developed DWL significantly improved bacterial identification rates in drinking water samples.
- The library offers faster identification compared to clinical MALDI-TOF MS databases.
- The DWL enhances the routine microbiological control capabilities for water operators.
Conclusions:
- A tailored MALDI-TOF MS drinking water library is effective for identifying bacteria in water intended for consumption.
- This specialized library supports regulatory compliance and improves water quality management.
- The DWL represents a valuable advancement for the microbiological surveillance of drinking and bottled water.
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