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Generation of Tissue Spheroids via a 3D Printed Stamp-Like Device
Published on: October 6, 2022
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3D bioprinted tissue-specific spheroidal multicellular microarchitectures for advanced cell therapy.
Yejin Park1, Seung Taek Ji2, Uijung Yong1
1Department of Convergence IT Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Kyungbuk 37673, Republic of Korea.
Biofabrication
|August 25, 2021
Summary
Engineered spheroidal multicellular microarchitectures (SMMs) enhance cell therapy. This 3D bioprinting method produces SMMs with high cell viability, improving tissue regeneration and cell function.
Area of Science:
- Biomaterials Engineering
- Tissue Engineering
- Regenerative Medicine
Background:
- Intercellular interactions are vital for cell viability and function in engineered tissues.
- Spheroidal multicellular microarchitectures (SMMs) offer enhanced cell-cell and cell-extracellular matrix (ECM) interactions.
- SMMs serve as building blocks and injectable carriers for cell therapy.
Purpose of the Study:
- To develop a precise and rapid 3D bioprinting method for creating SMMs.
- To fabricate SMMs using a bioink blend of decellularized ECM and alginate for enhanced cellular performance.
- To assess the potential of SMMs for cell therapy and tissue regeneration.
Main Methods:
- Utilized microextrusion-based 3D bioprinting technology.
- Developed an optimal bioink incorporating decellularized ECM and alginate.
- Fabricated size-controllable and mass-producible SMMs with encapsulated cells.
Main Results:
- Achieved high cell viability and mass production of SMMs.
- Demonstrated size controllability of the fabricated SMMs.
- Co-culture of SMMs with endothelial cells promoted lineage-specific maturation and increased functionality compared to monocultures.
Conclusions:
- SMMs exhibit potential for cell therapy due to high cell retention and proliferation.
- SMMs are particularly promising for efficient tissue regeneration, such as after myocardial infarction.
- 3D bioprinting of cell-niche-standardized SMMs expands applications in regenerative medicine.
