Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Therapeutic Levels of Hypothermia Achieved in Isolated Porcine Eyes Using a Scleral Contact Interface.

Annals of biomedical engineering·2025
Same author

Effects of normal aging on the mouse retina assessed by full-field flash and flicker electroretinography.

Scientific reports·2023
Same author

Three-Dimensional Model of Electroretinogram Field Potentials in the Rat Eye.

IEEE transactions on bio-medical engineering·2018
Same author

Three Dimensional Stimulus Source for Pattern Electroretinography in Mid- and Far-peripheral Retina.

Translational vision science & technology·2018
Same author

Corneal Potential Maps Measured With Multi-Electrode Electroretinography in Rat Eyes With Experimental Lesions.

Investigative ophthalmology & visual science·2017
Same author

Whole-eye electrical stimulation therapy preserves visual function and structure in P23H-1 rats.

Experimental eye research·2016

Related Experiment Video

Updated: Oct 23, 2025

Multiscale Investigations of Cortical Processing by Integrating Laminar Polytrodes and Optogenetics with Micro Electrocorticography in Rodents
07:52

Multiscale Investigations of Cortical Processing by Integrating Laminar Polytrodes and Optogenetics with Micro Electrocorticography in Rodents

Published on: May 23, 2025

466

Visualizing spatial differences in corneal electroretinogram potentials using a three-dimensional surface spline.

Brian E Kunzer1, Zahra Derafshi1, John R Hetling1

  • 1Richard and Loan Hill Department of Bioengineering, University of Illinois at Chicago, Chicago, IL 60607, United States of America.

Journal of Neural Engineering
|August 25, 2021
PubMed
Summary

A novel 3D surface spline method effectively interpolates multi-electrode electroretinography (meERG) data, creating clear corneal potential maps. This technique enhances visualization and analysis of retinal function, distinguishing healthy from diseased eyes.

Keywords:
ERGcorneaelectrophysiological imagingelectroretinograminterpolationmeERGmulti-electrode electroretinography

More Related Videos

Simultaneous Recording of Electroretinography and Visual Evoked Potentials in Anesthetized Rats
10:30

Simultaneous Recording of Electroretinography and Visual Evoked Potentials in Anesthetized Rats

Published on: July 1, 2016

12.6K
Correlative Confocal and 3D Electron Microscopy of a Specific Sensory Cell
08:00

Correlative Confocal and 3D Electron Microscopy of a Specific Sensory Cell

Published on: July 19, 2015

11.6K

Related Experiment Videos

Last Updated: Oct 23, 2025

Multiscale Investigations of Cortical Processing by Integrating Laminar Polytrodes and Optogenetics with Micro Electrocorticography in Rodents
07:52

Multiscale Investigations of Cortical Processing by Integrating Laminar Polytrodes and Optogenetics with Micro Electrocorticography in Rodents

Published on: May 23, 2025

466
Simultaneous Recording of Electroretinography and Visual Evoked Potentials in Anesthetized Rats
10:30

Simultaneous Recording of Electroretinography and Visual Evoked Potentials in Anesthetized Rats

Published on: July 1, 2016

12.6K
Correlative Confocal and 3D Electron Microscopy of a Specific Sensory Cell
08:00

Correlative Confocal and 3D Electron Microscopy of a Specific Sensory Cell

Published on: July 19, 2015

11.6K

Area of Science:

  • Ophthalmology
  • Neuroscience
  • Biomedical Engineering

Background:

  • The spatial distribution of retinal activity dictates corneal electroretinogram (ERG) potentials.
  • Multi-electrode electroretinography (meERG) generates complex datasets requiring advanced analysis for accurate interpretation.

Purpose of the Study:

  • To evaluate a 3D surface spline method for interpolating corneal potentials in meERG data.
  • To assess the effectiveness of this method in visualizing and analyzing retinal function, particularly local dysfunction.

Main Methods:

  • 25-channel meERG responses were recorded from rat eyes before and after induced local lesions.
  • A 3rd order surface spline was employed for interpolating meERG values and generating normalized, color-coded corneal potential maps.
  • Spatial standard deviation and difference-from-normal plots were used to characterize pre- and post-treatment responses.

Main Results:

  • Eyes with local lesions exhibited significantly higher spatial standard deviation compared to healthy eyes.
  • The 3rd order spline produced reliable corneal potential maps with low error rates, even with up to 30% channel loss.
  • Normalized responses clearly distinguished lesioned eyes from the healthy-eye mean, highlighting the sensitivity of spatial standard deviation to local dysfunction.

Conclusions:

  • A 3rd order surface spline is a suitable method for interpolating meERG potentials and creating corneal potential maps.
  • This approach addresses key challenges in meERG analysis, including visualization, normalization, and identification of abnormal responses.
  • The developed meERG analysis technique shows promise for research and clinical applications, especially in detecting early disease or therapeutic effects.