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Simple kinetic method for assessing catalase activity in biological samples.

Ali A Farman¹, Mahmoud Hussein Hadwan¹

¹Chemistry Dept., College of Science, University of Babylon, Hilla city, Babylon Governorate, p.o. 51002, Iraq.

|August 26, 2021

Summary

A new kinetic method accurately measures catalase activity in biological samples using hydrogen peroxide and pyrogallol red. This reliable assay is suitable for diagnostics and research.

Keywords:

Biological fluids Catalase activity Hydrogen peroxide Molybdenum Pyrogallol red

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Area of Science:

Biochemistry
Enzymology
Analytical Chemistry

Background:

Catalase is a crucial enzyme for cellular protection against oxidative stress.
Accurate measurement of catalase activity is vital for diagnosing various diseases and for research.
Existing methods for catalase activity assay may have limitations in speed, ease of use, or applicability.

Purpose of the Study:

To evaluate a novel kinetic method for quantifying catalase activity in biological samples.
To establish the reliability and precision of the new assay for diagnostic and research purposes.
To compare the novel method with an established technique for catalase measurement.

Main Methods:

A kinetic assay based on the oxidation of pyrogallol red by hydrogen peroxide, catalyzed by molybdenum.
Measurement of the decrease in pyrogallol red absorbance over time.
Validation using erythrocyte lysate homogenates and comparison with the ammonium peroxovanadate method.
Assessment of within-run and between-run coefficients of variation.

Main Results:

The method demonstrates a direct proportionality between the decrease in absorbance and hydrogen peroxide concentration, thus reflecting catalase activity.
Results obtained using the novel method showed significant correlation with the ammonium peroxovanadate method.
High precision was confirmed with coefficients of variation of 3.1% (within-run) and 4.7% (between-run).

Conclusions:

The novel kinetic method provides a rapid, easy, and reliable means for measuring catalase activity.
The assay is validated for various biological samples and shows high precision.
This method is suitable for application as a diagnostic tool and in research settings.