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Identification of Novel miRNAs in the F8 Gene Via Bioinformatics Tools
Halimeh Rezaei1, Majid Motovali-Bashi1, Sheyda Khalilian1
1Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Iran.
Background:
Hemophilia A is an X-linked bleeding disorder resulting in a deficiency of plasma clotting factor VIII and caused by mutations in the FVIII gene (F8 gene). MicroRNAs (miRNAs) in body fluids are promising biomarker candidates for Hemophilia A, due to their stability in body fluids and accessibility by non- or minimally-invasive procedures. Therefore; Advances in miRNA analysis methods resulted in a wide range of publications on miRNAs as putative biomarkers.
Objective:
Here we tried to scan the F8 gene region to predict a novel miRNA and identify it as a regulator of the F8 gene.
Materials And Methods:
To this aim, the ability to express novel miRNAs in F8 locus was assessed via reliable bioinformatics databases such as SSCprofiler, RNAfold, miREval, FOMmiR, MaturBayes, miRFIND, UCSC genome browser, Deep Sequencing, and miRBase.
Results:
Data analysis from the relevant databases offers one stem-loop structure that is predicted to express a novel miRNA.
Conclusions:
The diagnosis of Hemophilia A with the help of these types of biomarkers is a non-invasive procedure that has been demonstrated to have a significant role in the early diagnosis of the disease. Hopefully, the proposed candidate sequence will be confirmed in vitro and become a non-invasive biomarker in the near future.
Insights
Researchers identified a novel microRNA candidate within the F8 gene region, potentially serving as a non-invasive biomarker for Hemophilia A diagnosis. This discovery could lead to earlier disease detection through accessible body fluid analysis.
Area of Science:
- Genetics and Molecular Biology
- Biomarker Discovery
- Bioinformatics
Background:
- Hemophilia A is an X-linked bleeding disorder caused by Factor VIII deficiency due to F8 gene mutations.
- MicroRNAs (miRNAs) are stable in body fluids, making them promising non-invasive biomarkers for Hemophilia A.
- Advancements in miRNA analysis have spurred research into their potential as diagnostic markers.
Purpose of the Study:
- To scan the F8 gene region for novel miRNA prediction.
- To identify a novel miRNA that regulates the F8 gene.
Main Methods:
- Utilized bioinformatics databases including SSCprofiler, RNAfold, miREval, FOMmiR, MaturBayes, miRFIND, UCSC genome browser, Deep Sequencing, and miRBase.
- Assessed the potential for novel miRNA expression within the F8 locus.
Main Results:
- Data analysis identified a single stem-loop structure.
- This structure is predicted to express a novel miRNA.
Conclusions:
- The identified miRNA candidate holds potential for non-invasive Hemophilia A diagnosis.
- Further in vitro confirmation is needed to establish this miRNA as a reliable biomarker for early disease detection.
