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Updated: Oct 22, 2025

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
Published on: March 8, 2018
The complete chloroplast genome sequence of Brachypodium sylvaticum
Yunqiang Wang1,2, Yi Wang2, Xiaolong Yuan2
1Yunnan Branch, Institute of Medicinal Plant, Chinese Academy of Medical Sciences, Yunnan Key Laboratory of Southern Medicinal Resources, Kunming, PR China.
Abstract:
The first complete chloroplast genome (cpDNA) sequence of Brachypodium sylvaticum was determined from Illumina HiSeq pair-end sequencing data in this study. The cpDNA is 136,392 bp in length, contains a large single-copy region (LSC) of 80,854 bp and a small single-copy region (SSC) of 12,765 bp, which were separated by a pair of inverted repeats (IR) regions of 21,383 bp. The genome contains 130 genes, including 84 protein-coding genes, 8 ribosomal RNA genes, and 38 transfer RNA genes. Further phylogenomic analysis showed that B. sylvaticum and B. distachyon clustered in a unique clade in Brachypodium genus.
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