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lncRNA - Long Non-coding RNAs02:39

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
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The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
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Updated: Oct 22, 2025

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation
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LncRNA00492 is required for marginal zone B-cell development.

Faming Wang1, Dongya Cui2,3, Qingyun Zhang4

  • 1Department of Biochemistry and Molecular Biology, Medical School of Southeast University, Nanjing, China.

Immunology
|August 26, 2021
PubMed
Summary

Long non-coding RNA Gme00492 is crucial for B-cell development. It regulates marginal zone B cells by interacting with CTBP1, impacting Notch2 signaling and preventing developmental blocks.

Keywords:
B-cell developmentCTBP1Lnc00492marginal zone B cellsnotch2 signalling

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Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • B-cell development is a complex process essential for immune function.
  • Defects in B-cell development can result in immunodeficiency, leukemia, or lymphoma.
  • Long non-coding RNAs (lncRNAs) are emerging as critical regulators in biological processes, but their role in B-cell development is largely unknown.

Purpose of the Study:

  • To investigate the expression and function of lncRNA Gme00492 during B-cell development.
  • To elucidate the molecular mechanisms by which lncRNA Gme00492 influences B-cell differentiation.
  • To identify potential therapeutic targets for B-cell malignancies.

Main Methods:

  • Analysis of lncRNA Gme00492 expression in developing B cells.
  • Generation and analysis of lncRNA Gme00492-deficient mouse models.
  • Co-immunoprecipitation and ubiquitination assays to study protein interactions.
  • Assessment of B-cell populations in the spleen and bone marrow.

Main Results:

  • lncRNA Gme00492 is highly expressed during B-cell development and localized to the nucleus.
  • Mice deficient in lncRNA Gme00492 exhibit a significant reduction in marginal zone B cells.
  • lncRNA Gme00492 directly interacts with CTBP1, promoting its ubiquitination and degradation.
  • CTBP1 is a critical regulator of Notch2 signaling during B-cell development.

Conclusions:

  • lncRNA Gme00492 is essential for the proper development of marginal zone B cells.
  • A novel regulatory axis involving lncRNA Gme00492 and CTBP1 controls B-cell differentiation via Notch2 signaling.
  • Dysregulation of this axis may contribute to B-cell malignancies.