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Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...

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Related Experiment Video

Updated: Oct 22, 2025

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Published on: April 16, 2016

VEGF Detection via Simplified FLISA Using a 3D Microfluidic Disk Platform.

Dong Hee Kang¹, Na Kyong Kim¹, Sang-Woo Park²

¹Department of Mechanical Engineering, Chonnam National University, 77 Youngbong-ro, Buk-Gu, Gwangju 61186, Korea.

|August 26, 2021

Summary

This summary is machine-generated.

This study introduces a novel 3D microfluidic disk for fluorescence-linked immunosorbent assay (FLISA), significantly reducing reagent use and assay time. The system automates sample processing for sensitive detection of biomarkers like vascular endothelial growth factor.

Keywords:

3D microstructure fluorescence-linked immunosorbent assay lab-on-a-disk vascular endothelial growth factor

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A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors

A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors

Published on: March 15, 2016

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Last Updated: Oct 22, 2025

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Published on: April 16, 2016

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors

A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors

Published on: March 15, 2016

Area of Science:

Biomedical Engineering
Analytical Chemistry
Biochemistry

Background:

Fluorescence-linked immunosorbent assay (FLISA) is a standard quantitative method for detecting biochemical changes via antigen-antibody interactions on well-plate platforms.
Advancements in microfluidic systems enable the implementation of FLISA on microfluidic disks for high-resolution detection of trace biochemical reactions.

Purpose of the Study:

To develop a novel microfluidic system for enhanced FLISA performance.
To reduce reagent consumption and assay time for biochemical detection.

Main Methods:

A microfluidic disk with a unique three-dimensional incubation chamber was designed and fabricated.
The system utilizes centrifugal and gravitational forces for automated control of antibody-conjugated microbead movement during incubation.
Vascular endothelial growth factor (VEGF) was detected sequentially using the 3D microfluidic disk FLISA protocol.

Main Results:

The 3D microfluidic disk reduced reagent volume by 1/10 and assay time to under one hour.
Automated microbead movement within the 3D chamber ensured efficient antigen-antibody and substrate binding.
The system demonstrated effective sequential detection of VEGF concentrations in ng/mL without manual intervention.

Conclusions:

The proposed 3D microfluidic disk offers a highly efficient and automated platform for FLISA.
This technology significantly enhances sensitivity and reduces resource requirements for biochemical assays.
The system holds potential for point-of-care diagnostics and high-throughput screening applications.