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High-performance liquid chromatographic method for determining trichothecene mycotoxins by post-column fluorescence
A Sano1, S Matsutani, M Suzuki
1Faculty of Pharmaceutical Sciences, Science University of Tokyo, Japan.
Journal of Chromatography
|December 11, 1987
Summary
This study presents a new method for detecting trichothecenes like deoxynivalenol in grains. The fluorescence detection method achieves high recoveries, ensuring accurate mycotoxin analysis in food safety.
Area of Science:
- Analytical Chemistry
- Food Chemistry
- Mycology
Background:
- Trichothecenes, such as deoxynivalenol (DON), are common mycotoxins in cereals.
- Accurate quantification of these toxins is crucial for food safety and regulatory compliance.
Purpose of the Study:
- To develop and validate a sensitive method for determining deoxynivalenol, nivalenol, and fusarenon-X in cereal matrices.
- To establish efficient sample preparation techniques for reliable mycotoxin analysis.
Main Methods:
- Separation of trichothecenes using a C18 column with aqueous acetonitrile.
- Post-column fluorescence derivatization employing alkaline decomposition and a modified Hantzsch reaction.
- Sample clean-up utilizing a Florisil column and a Sep-Pak CN cartridge.
Main Results:
- The method allows for the determination of trichothecenes at levels of 5-10 ng.
- Recoveries for deoxynivalenol and nivalenol in spiked corn, wheat, and barley ranged from 61.4% to 96.9% at 0.05-1 ppm.
- The fluorescence detection system operated with excitation and emission wavelengths of 370 nm and 460 nm, respectively.
Conclusions:
- The developed method provides a reliable and sensitive approach for quantifying key trichothecenes in cereal grains.
- The combination of chromatographic separation, fluorescence detection, and effective clean-up ensures accurate mycotoxin assessment in food products.