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Updated: Aug 14, 2026

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis
Published on: July 11, 2014
Determination of oxpentifylline and three metabolites in plasma by automated capillary gas chromatography using
1Hoechst Pharmaceutical Research Laboratories, Walton, Milton Keynes.
Abstract:
A procedure is described for the simultaneous determination of oxpentifylline and three metabolites in plasma by capillary gas chromatography. Plasma samples are acidified and extracted with chloroform. The carboxylic acid compounds are converted to their methyl esters and the hydroxylated metabolites to their O-trifluoroacetates prior to analysis by capillary gas chromatography using nitrogen-selective detection. The detection limits for the compounds are 5 ng/ml and the assay has been applied to the analysis of plasma from volunteer trials after oral administration of oxpentifylline.

