Solid-phase extraction and high-performance liquid chromatographic method for chlorpromazine and thirteen metabolites

Area of Science:

• Pharmacology
• Analytical Chemistry
• Clinical Chemistry

Background:

• Chlorpromazine is a widely used antipsychotic medication.
• Monitoring chlorpromazine levels and its metabolites is crucial for effective treatment and patient safety.
• Existing methods for quantifying chlorpromazine and its metabolites may lack speed, sensitivity, or reliability.

Purpose of the Study:

• To develop and validate a rapid and reliable analytical method for determining chlorpromazine and its common metabolites in human plasma.
• To establish a sensitive assay capable of quantifying analytes at the nanogram per milliliter concentration range.
• To assess the method's performance characteristics, including recovery, detection limits, and reproducibility.

Main Methods:

• Solid-phase extraction using a C8 bonded phase for sample preparation.
• Reversed-phase isocratic high-performance liquid chromatography (HPLC) for separation.
• Quantitation using an internal standard.

Main Results:

• A robust method was established for the simultaneous determination of chlorpromazine and thirteen metabolites.
• The method demonstrated high sensitivity, with quantitation achievable in the ng/ml range in human plasma.
• Evaluation confirmed good recovery, low detection limits, and high reproducibility, indicating reliability for clinical application.

Conclusions:

• The developed C8 SPE-HPLC method provides a rapid, reliable, and sensitive approach for quantifying chlorpromazine and its metabolites in human plasma.
• This method is suitable for therapeutic drug monitoring and pharmacokinetic studies.
• The validated assay can be applied to analyze patient samples, supporting clinical decision-making and research.