Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

10.6K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
10.6K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

476
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
476
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

757
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
757

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Crohn's Disease in Malaysia: Could Application of the Precautionary Principle Reduce Future Incidence?

Microorganisms·2026
Same author

Geographic distribution of inflammatory bowel disease in the UK: A spatially explicit survey.

PloS one·2025
Same author

<i>Leishmania mexicana</i> pathogenicity requires flagellar assembly but not motility.

Virulence·2025
Same author

A phase 1b clinical trial to determine the safety, tolerability and immunogenicity of simian adenovirus and poxvirus vectored vaccines against a Mycobacterium avium complex subspecies in patients with active Crohn's disease.

EBioMedicine·2025
Same author

Corrigendum to "Visualisation of Mycobacterium avium subsp. paratuberculosis in cultured cells, infected sheep and human tissue sections using fluorescent in situ hybridization (FISH)" [Journal of Microbiological Methods 224 (2024) 107001].

Journal of microbiological methods·2024
Same author

Visualisation of Mycobacterium avium subsp. paratuberculosis in cultured cells, infected sheep and human tissue sections using fluorescent in situ hybridization (FISH).

Journal of microbiological methods·2024
Same journal

A Novel Laboratorial Approach to Evaluate Bacterial Microleakage of Endodontic Sealers.

Current protocols·2026
Same journal

TRIAGE Toolkit: Streamlined Discovery of Regulatory Genes and Elements.

Current protocols·2026
Same journal

High-throughput Profiling of Pseudouridines in Microbiome-derived Bacterial RNA.

Current protocols·2026
Same journal

Recombinant Protein Expression in Rhodococcus species.

Current protocols·2026
Same journal

Streamlined In Vitro Transcription for Generating Self-Amplifying RNA With Modified Nucleotides.

Current protocols·2026
Same journal

CODEC Library Preparation From Genomic DNA.

Current protocols·2026
See all related articles

Related Experiment Video

Updated: Oct 22, 2025

Micron-scale Resolution Optical Tomography of Entire Mouse Brains with Confocal Light Sheet Microscopy
09:49

Micron-scale Resolution Optical Tomography of Entire Mouse Brains with Confocal Light Sheet Microscopy

Published on: October 8, 2013

16.9K

Super-Resolution Fluorescence Microscopy Methods for Assessing Mouse Biology.

Jessica Valli1, Jeremy Sanderson2

  • 1Edinburgh Super Resolution Imaging Consortium (ESRIC), Institute of Biological Chemistry, Biophysics and Bioengineering, Heriot-Watt University, Edinburgh, United Kingdom.

Current Protocols
|August 26, 2021
PubMed
Summary
This summary is machine-generated.

Super-resolution microscopy, a Nobel Prize-winning technique, enhances light microscopy for diverse biological applications. This review covers key methods and guides researchers in selecting the best super-resolution approach for their studies.

Keywords:
pixel reassignmentsingle molecule localization microscopystimulated emission depletion microscopystructured illumination microscopysuper resolution microscopysuper resolution radial fluctuations

More Related Videos

Visualization of Motor Axon Navigation and Quantification of Axon Arborization In Mouse Embryos Using Light Sheet Fluorescence Microscopy
08:56

Visualization of Motor Axon Navigation and Quantification of Axon Arborization In Mouse Embryos Using Light Sheet Fluorescence Microscopy

Published on: May 11, 2018

7.9K
Light-sheet Fluorescence Microscopy for the Study of the Murine Heart
08:42

Light-sheet Fluorescence Microscopy for the Study of the Murine Heart

Published on: September 15, 2018

9.5K

Related Experiment Videos

Last Updated: Oct 22, 2025

Micron-scale Resolution Optical Tomography of Entire Mouse Brains with Confocal Light Sheet Microscopy
09:49

Micron-scale Resolution Optical Tomography of Entire Mouse Brains with Confocal Light Sheet Microscopy

Published on: October 8, 2013

16.9K
Visualization of Motor Axon Navigation and Quantification of Axon Arborization In Mouse Embryos Using Light Sheet Fluorescence Microscopy
08:56

Visualization of Motor Axon Navigation and Quantification of Axon Arborization In Mouse Embryos Using Light Sheet Fluorescence Microscopy

Published on: May 11, 2018

7.9K
Light-sheet Fluorescence Microscopy for the Study of the Murine Heart
08:42

Light-sheet Fluorescence Microscopy for the Study of the Murine Heart

Published on: September 15, 2018

9.5K

Area of Science:

  • Biophysics
  • Cell Biology
  • Microscopy

Background:

  • Super-resolution microscopy, developed 15 years ago, has revolutionized light microscopy.
  • Its developers received the Nobel Prize in Chemistry in 2014.
  • The technique is now widely used across various scientific fields, from molecular to organismal levels.

Purpose of the Study:

  • To review the principles and applications of super-resolution microscopy.
  • To explain how it has contributed to the advancement of light microscopy.
  • To guide researchers in choosing appropriate super-resolution techniques.

Main Methods:

  • Structured Illumination Microscopy (SIM)
  • Super-resolution Radial Fluctuations (SRRF)
  • Stimulated Emission Depletion (STED) microscopy
  • Single-Molecule Localization Microscopy (SMLM)
  • Super-resolution Imaging by Pixel Reassignment (iPR)

Main Results:

  • Super-resolution microscopy enables imaging beyond the diffraction limit, offering unprecedented detail.
  • The review details various super-resolution techniques, including their underlying principles.
  • It highlights the broad applicability of these methods in biological research.

Conclusions:

  • Super-resolution microscopy has revitalized light microscopy, offering significant advantages for biological research.
  • Understanding the strengths and weaknesses of each technique is crucial for effective experimental design.
  • This review provides a comprehensive guide for selecting the optimal super-resolution method.