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Updated: Oct 22, 2025

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
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Controlled DNA Delivery Using Poly(lactide) Nanoparticles and Understanding the Binding Interactions.

Sudipta Senapati1, Anurag Upadhyaya2, Somnath Dhruw1

  • 1School of Materials Science and Technology, Indian Institute of Technology (Banaras Hindu University), Varanasi 221 005, India.

The Journal of Physical Chemistry. B
|August 26, 2021
PubMed
Summary
This summary is machine-generated.

This study introduces poly(l-lactide) nanoparticles as a novel gene delivery vector with high DNA loading and controlled release. Molecular simulations reveal atomic-level interactions, ensuring DNA protection and efficient delivery.

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Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Molecular Biology

Background:

  • Cationic polymers for gene delivery face limitations like low DNA loading, poor transfection, toxicity, and environmental instability.
  • Detailed atomic-level understanding of binding interactions is scarce, hindering optimization of DNA loading, protection, and release.

Purpose of the Study:

  • To develop a poly(l-lactide) (PLA) nanoparticle-based system for controlled DNA release.
  • To investigate the atomic-level interactions between PLA and DNA to understand high loading, environmental protection, and controlled release.

Main Methods:

  • Spectroscopic and physicochemical characterization.
  • Molecular simulations including AM1 and atomistic molecular dynamics.
  • Methyl thiazolyl tetrazolium (MTT) assay for biocompatibility.
  • Cellular uptake and endo-lysosomal escape studies in HeLA cells.

Main Results:

  • The developed PLA nanoparticles exhibit high DNA-loading capacity.
  • Controlled DNA release was achieved.
  • Molecular simulations elucidated binding interactions responsible for DNA encapsulation and protection.
  • MTT assays confirmed the biocompatibility of the PLA nanoparticles.
  • Efficient cellular uptake and endo-lysosomal escape were observed in HeLA cells.

Conclusions:

  • Poly(l-lactide) nanoparticles represent a promising nonviral DNA delivery vector.
  • Detailed atomic-level interaction studies provide insights into the mechanism of DNA loading, protection, and release.
  • This system offers potential for improved gene therapy applications.