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Updated: Aug 5, 2026

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Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects
Published on: February 18, 2014
Characterization of biotinylated proteins in mammalian cells using 125I-streptavidin
1Sir William Dunn School of Pathology, Oxford, U.K.
Journal of Biochemical and Biophysical Methods
|December 1, 1987
Abstract:
The presence of biotinylated proteins in detergent extracts of cells is demonstrated using 125I-streptavidin. Four components with apparent molecular masses (Mr) ranging from 70 to 220 kDa have been detected. These probably represent the biotinylated subunits of the carboxylase enzymes. The proportions of the four components were found to vary from cell type to cell type and also the Mr varied from species to species.

