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Identification of Post-translational Modifications of Plant Protein Complexes

Identification of Post-translational Modifications of Plant Protein Complexes

Published on: February 22, 2014

Multilayer regulatory landscape during pattern-triggered immunity in rice.

Bozeng Tang¹, Caiyun Liu², Zhiqiang Li¹

¹State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.

Plant Biotechnology Journal

|August 26, 2021

Summary

Rice plants activate pattern-triggered immunity (PTI) against fungal and bacterial pathogens. Multi-omics analysis reveals conserved and distinct cellular responses, highlighting post-translational modifications in rice defense.

Keywords:

PAMPs PTI-mediated defence multi-omics plant innate immunity

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Area of Science:

Plant immunity
Molecular biology
Agricultural science

Background:

Plants defend against pathogens via pattern-triggered immunity (PTI) upon recognizing pathogen-associated molecular patterns (PAMPs).
A comprehensive understanding of cellular reprogramming during PTI using multi-omics is needed.

Purpose of the Study:

To investigate rice (Oryza sativa) PTI responses to fungal (chitin) and bacterial (flg22) PAMPs.
To systematically analyze cellular processes during PTI using integrated multi-omics data.

Main Methods:

Generated metabolome, transcriptome, proteome, ubiquitome, and acetylome data for rice.
Performed integrative multi-omics analysis to compare responses to chitin and flg22.

Main Results:

Identified convergence and divergence in rice responses to chitin and flg22 at multiple regulatory levels.
Observed similar transcriptome and proteome responses but distinct metabolome profiles.
Post-translational modifications (ubiquitination, acetylation) likely mediate differential metabolite profiles and enzyme activity.

Conclusions:

Multi-omics analysis provides a regulatory atlas of metabolic pathways, including defense-related biosynthesis.
This study establishes a foundation for dissecting PTI mechanisms in rice and related crops.