Stable isotope and photosynthetic response of tea grown under different temperature and light conditions

The stable isotope and photosynthesis response of tea (Camellia sinensis) is determined under different light and temperature conditions. The results showed that isotopes of young tea leaves were more enriched with increasing light intensity (31 ~ 411 µmol m^-2∙s^-1). However, the value of δ¹³C and δ¹⁵N seemed depleted, while δ²H and δ¹⁸O became enriched as temperature increasing from 15 to 35 °C. Significant isotope differences were found in tea leaves harvested between early growth (0 ~ 10 days) and later growth (10 ~ 21 days) periods (p < 0.05). Pearson's correlation showed a negative correlation between isotopes (δ¹³C, δ¹⁵N and δ²H) and photosynthetic parameters (EVAP and CI) ranging from 0.497 to 0.872, under 25 °C/203 µmol m^-2∙s^-1. But δ¹⁸O had a weak correlation with all photosynthetic parameters under the same conditions. These distinctive correlations between isotopes and photosynthetic parameters provide new insights which could be used to predict tea isotope responses arising from subtle seasonal or climate change conditions.