Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effects of Marijuana Use on Female Reproductive Health and Pregnancy.

Contemporary ob/gyn·2026
Same author

Generation of a rhesus macaque harboring a multivalent reporter for assessing gene editing outcomes.

Scientific reports·2026
Same author

Complex Substrates and Bedding for Environmental Enhancement and Research: Or, the Nature of Primates.

American journal of primatology·2026
Same author

Ethological scars? Exposure to multiple negative events over a lifespan may predict abnormal repetitive behaviour in laboratory-housed rhesus macaques.

Biology letters·2026
Same author

Non-human primates as a translational model for the study of male reproductive health.

Nature reviews. Urology·2025
Same author

Ultrasound-Guided Rete Testis Approach to Sperm Aspiration and Spermatogonial Stem Cell Transplantation in Patients with Azoospermia.

medRxiv : the preprint server for health sciences·2025

Related Experiment Video

Updated: Oct 22, 2025

Laparoscopic Technique for Serial Collection of Liver and Mesenteric Lymph Nodes in Macaques
11:06

Laparoscopic Technique for Serial Collection of Liver and Mesenteric Lymph Nodes in Macaques

Published on: May 2, 2017

51.3K

Improved Training and Semen Collection Outcomes Using the Closed Box Chair for Macaques.

Lisa A Houser1, Cathy Ramsey2, Fernanda M de Carvalho2

  • 1Oregon National Primate Research Center, Division of Comparative Medicine, Oregon Health & Science University, Beaverton, OR 97006, USA.

Animals : an Open Access Journal From MDPI
|August 27, 2021
PubMed
Summary

The closed box chair (CBC) is a more effective and less stressful method for collecting monkey semen than the open restraint chair (ORC). This refinement improves animal welfare and enhances semen quality for research and conservation.

Keywords:
refinementrestraintsemen volumesperm concentrationstresswelfare

More Related Videos

Author Spotlight: Training of Laboratory Animals for Gentle and Stress-Free Handling
05:21

Author Spotlight: Training of Laboratory Animals for Gentle and Stress-Free Handling

Published on: February 16, 2024

2.9K
Author Spotlight: Marmoset Research - Scope and Challenges
04:52

Author Spotlight: Marmoset Research - Scope and Challenges

Published on: June 9, 2023

2.1K

Related Experiment Videos

Last Updated: Oct 22, 2025

Laparoscopic Technique for Serial Collection of Liver and Mesenteric Lymph Nodes in Macaques
11:06

Laparoscopic Technique for Serial Collection of Liver and Mesenteric Lymph Nodes in Macaques

Published on: May 2, 2017

51.3K
Author Spotlight: Training of Laboratory Animals for Gentle and Stress-Free Handling
05:21

Author Spotlight: Training of Laboratory Animals for Gentle and Stress-Free Handling

Published on: February 16, 2024

2.9K
Author Spotlight: Marmoset Research - Scope and Challenges
04:52

Author Spotlight: Marmoset Research - Scope and Challenges

Published on: June 9, 2023

2.1K

Area of Science:

  • Primate reproductive biology
  • Animal welfare science
  • Conservation science

Background:

  • Semen collection in non-human primates is crucial for research, management, and conservation.
  • The traditional open restraint chair (ORC) with pole and collar can cause stress and anxiety in some individuals.
  • Refining restraint techniques is essential for improving animal welfare during sample collection.

Purpose of the Study:

  • To compare the efficacy and animal welfare outcomes of the closed box chair (CBC) versus the open restraint chair (ORC) for semen collection in rhesus macaques.
  • To evaluate the impact of restraint method on training success, training time, and semen quality parameters.

Main Methods:

  • Thirty-four adult male rhesus macaques were trained using positive reinforcement to provide semen samples via either ORC or CBC.
  • Training success rates and time to train were recorded for both methods.
  • Ejaculatory volume, sperm concentration, and sperm motility were compared between males sampled using ORC and CBC.

Main Results:

  • Training success was significantly higher in the CBC group (100%) compared to the ORC group (75%) (p=0.04).
  • Training time was significantly shorter for the CBC method (201.0 min) than the ORC method (412.4 min) (p<0.001).
  • Males collected via CBC yielded significantly higher ejaculatory volume (522.1 µL vs. 297.6 µL; p=0.04) and sperm concentration (367.5 × 10^6/mL vs. 186.0 × 10^6/mL; p=0.017) than those collected via ORC.

Conclusions:

  • The closed box chair (CBC) method is a more efficient and effective technique for training and semen collection in rhesus macaques.
  • The CBC reduces animal stress and enhances key semen quality parameters, supporting its use as a welfare refinement.
  • This refined technique has significant implications for improving animal welfare and data quality in primate research and conservation programs.