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Updated: Oct 22, 2025

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Three-Dimensional Vascularized Lung Cancer-on-a-Chip with Lung Extracellular Matrix Hydrogels for In Vitro Screening.

Sangun Park1,2, Tae Hee Kim1, Soo Hyun Kim1,3

  • 1Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology (KIST), Seoul 02792, Korea.

Cancers
|August 27, 2021
PubMed
Summary

Researchers developed a 3D vascularized lung cancer-on-a-chip (VLCC) using decellularized lung extracellular matrix. This platform demonstrated enhanced Doxorubicin drug screening efficacy compared to traditional 2D methods.

Keywords:
angiogenesiscancer-on-a-chipdecellularized extracellular matrixdrug screeningtumor microenvironmentvascularization

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Area of Science:

  • Biomedical Engineering
  • Cancer Research
  • Drug Discovery

Background:

  • Advances in cancer immunotherapies and targeted therapies necessitate better in vitro models.
  • Simulating the tumor microenvironment, including vascularization, is crucial for understanding anti-cancer drug mechanisms.
  • Decellularized lung extracellular matrix (ldECM) provides a natural scaffold for vascularization and microenvironment mimicry.

Purpose of the Study:

  • To develop a 3D vascularized lung cancer-on-a-chip (VLCC) model.
  • To investigate the potential of ldECM-based hydrogels for creating perfusable vascular structures.
  • To evaluate the VLCC as a platform for anti-cancer drug screening.

Main Methods:

  • Fabrication of an ldECM-based hydrogel.
  • Encapsulation of tri-cellular spheroids (A549 cells, HUVECs, human lung fibroblasts) within the hydrogel.
  • Incorporation of perfusable microchannels to mimic vascular structures.
  • Assessment of Doxorubicin efficacy using the VLCC compared to 2D screening.

Main Results:

  • Successful development of a 3D VLCC model mimicking solid lung cancer.
  • Demonstration of perfusable vessel-like channels within the hydrogel construct.
  • Observed a more effective dose-dependent action of Doxorubicin using the VLCC compared to 2D screening.

Conclusions:

  • The VLCC serves as a promising 3D in vitro model for simulating lung cancer.
  • The ldECM-based hydrogel effectively supports vascularization and microenvironment mimicry.
  • The VLCC platform shows significant potential for improved 3D in vitro drug screening of anti-cancer therapies.